内质网蛋白MoPer1及其互作蛋白调控稻瘟病菌生长发育和致病的分子机制研究

项目名称： 内质网蛋白MoPer1及其互作蛋白调控稻瘟病菌生长发育和致病的分子机制研究

项目编号： No.31501599

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 陈岳

作者单位： 湖南省农业科学院

项目金额： 20万元

中文摘要： 丝状真菌细胞壁对维持其菌丝的正常形态以及适应环境具有非常重要的作用。GPI锚定是细胞壁蛋白转录翻译后糖基化磷脂酰肌醇修饰过程，在真核生物中这一过程非常普遍，且对于细胞壁的完整性是必需的，但是GPI锚定相关蛋白在植物病原真菌中的功能以及作用机制仍不清楚。本研究拟采用遗传学手段获得GPI锚定蛋白脂质重构过程中的关键基因MoPER1的敲除突变体，测定突变体细胞壁的完整性、生长发育、附着胞形成及致病性等生物学表型；进一步鉴定MoPer1互作蛋白，研究1-2个互作蛋白在稻瘟病菌细胞壁完整性、生长发育和致病中的作用。研究结果有助于阐明该GPI锚定相关的MoPer1及其互作蛋白调控的细胞壁完整性在稻瘟病菌发育、产孢及致病相关过程中的分子作用机制，同时为开发以调控细胞壁完整性的基因网络为靶标的防治稻瘟病的新型药剂提供新的线索。

中文关键词： 稻瘟病；生长发育；产孢机制；致病机理；调控网络

英文摘要： Filamentous fungal cell wall plays an essential role in maintaining hyphal morphology and adaptation to the environment. GPI-anchoring is a prevalent Glycosylphosphatidylinositol modification process of posttranslational protein and is necessary for cell wall integrity in eukaryotes. To date, the process and function of GPI anchored-related protein is still not known in phytopathogenic fungi. Here, we use genetics means to obtain the deletion mutant of MoPER1 that plays an important role in lipid remodeling of GPI-anchored proteins, and examine a series of biological phenotypes including cell wall integrity, growth, development, appressorium formation and pathogenicity. Furthermore, we identify the interactional protein of MoPer1 and elucidate the biological functions of one or two interactional proteins. The results will help to deep elucidate the molecular mechanism of cell wall integrity regulated by GPI-anchored-related protein MoPer1 and its interactional proteins during growth, development and pathogenicity of M. oryzae, and provide new clue for developing effective fungicide targeting genes regulation network involved in cell wall integrity to prevent the disease.

英文关键词： Magnaporthe oryzae;development;conidiogenesis;pathogenesis;regulating network