早期日粮能量水平调控肉仔鸡小肠磷转运的分子机制

项目名称： 早期日粮能量水平调控肉仔鸡小肠磷转运的分子机制

项目编号： No.31301992

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 李建慧

作者单位： 山西农业大学

项目金额： 24万元

中文摘要： 对肉仔鸡进行限制饲喂可缓解因生长速度过快引起的肉鸡不良反应。申请者在前期研究中发现通过对日粮营养浓度进行稀释可以控制生长速度，还可避免因限饲导致的饥饿,但日粮低营养浓度却引起肉仔鸡小肠磷转运吸收下降，其机制有待于进一步研究。另外前人研究表明，无机磷转运受肠道上皮细胞能量代谢水平的调控；AMPK 作为细胞重要的能量感受器，在调节能量代谢平衡的同时对肠上皮NaPi-2b的mRNA表达和蛋白表达也有调控。本课题拟分析肉仔鸡日粮能量和磷水平在调控磷转运过程中对小肠磷转运和AMPK信号的影响，继而通过体外试验用AMPK的促进剂对肠道细胞进行刺激，研究AMPK信号与肠道磷转运吸收的关系，最终明确AMPK通路是否在日粮能量调控磷吸收的过程中发挥作用。本研究将为理解肠道磷的转运机制奠定理论基础。

中文关键词： 肉仔鸡；能量水平；小肠磷转运；分子机制；

英文摘要： Feed restriction to broilers could alleviate a series of diseases due to high growth rate. My previous study found that low dietary nutrient density could not only control the growth rate of broilers, but also avoid the starvation induced by feed restriction, whereas in the meanwhile, the phosphate transport in small intestine of broilers was also declined with the mechanism unknown. Former research had showed that NaPi-2b was regulated by energy levels in enterocyte. AMP-activated protein kinase (AMPK), a sensor of peripheral energy balance, could play a role in regulating small epithelial cell NaPi-2b mRNA and protein expression. In the study， we will first analyze the small intestinal phosphate transport and AMPK signal pathway. Then an in vitro trial will be carried out with the activator of the AMPK added to reveal the relationship between intestinal phosphate transport and AMPK signal pathway. This study will provide a theoretical basis for understanding the intestinal phosphate transport mechanism.

英文关键词： broiler chickens；energy level；intestinal phosphate transport；molecular mechanism；