鸡线粒体全基因组DNA异质性及其潜在效应

项目名称： 鸡线粒体全基因组DNA异质性及其潜在效应

项目编号： No.31272434

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 黄艳群

作者单位： 河南农业大学

项目金额： 80万元

中文摘要： 线粒体异质性长期被认为呈阈值效应，十分稀有，仅出现在相应的病人中。但近年发现，人类线粒体DNA异质性具有普遍性。目前关于鸡线粒体异质性的研究尚未见报道。本课题组已发现鸡线粒体G4580A位点存在异质性并显示了潜在的重要效应。本项目拟在此基础上：①采用Solexa高通量测序，进行鸡腿肌组织线粒体DNA(mtDNA)全基因组异质性的鉴定及特性分析；②对鉴定的异质性变异位点，采用等位基因特异荧光定量PCR(ASP-qPCR)，研究mtDNA异质性的组织/品种分布特性；③利用固始鸡资源群体,研究异质性位点等位基因的传递特性；④利用固始鸡资源群F2 代个体,进行mtDNA异质性与相关性状/相应基因表达量的关联分析。以掌握鸡线粒体基因组异质状况、异质特性及其潜在效应。该研究可为利用mtDNA异质性进行鸡的分子选育和种系发育研究奠定基础。

中文关键词： 鸡；线粒体DNA；异质性；性状关联；基因表达

英文摘要： In a long time, mitochondrial heteroplasmy was thought to be rare, and only to be found in patients with a phenomenon referred to as the threshold effect (a minimum number of mutant genomes were required for the expression of disease).The recent study shows that human mitochondrial heteroplasmy is one common phenomenon while not one rare case. Up till now there were no reports about chicken mitochondrial heteroplasmy. In our lab, mitochondrial G4580A variant was found to be a heteroplasmic variation site and have potential important effect on some traits recently. Based on the preliminary findings, we plan to further conduct the following study: ① identify chicken mitochondrial DNA(mtDNA) heteroplasmy from the leg tissue in whole genomic mtDNA level by Solexa high throughput sequencing method; ②Based on the identified mitochondrial heteroplasmic sites, conduct the study about the tissue/breed distribution characteristics of these heteroplasmic sites with allele specific fluorescence quantitative PCR method (ASP-qPCR); ③conduct the study about the transmission characteristics of the allele for these heteroplasmic sites with the Gushi chicken resource population; ④conduct the association analysis between the mtDNA heteroplasmy of these sites and the related traits/ gene expression with F2 individuals of Gushi chi

英文关键词： Chicken；Mitochondrial DNA；Heteroplasmy；trait association analysis；Gene expression