基于微流控芯片快速检测海洋赤潮毒素新方法的研究

项目名称： 基于微流控芯片快速检测海洋赤潮毒素新方法的研究

项目编号： No.21207080

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 环境化学

项目作者： 程永强

作者单位： 山东省科学院

项目金额： 25万元

中文摘要： 针对海产品赤潮毒素研究领域，本项目以微流控芯片分析技术为基础，发展一种适用于赤潮毒素快速、高灵敏检测的新方法。项目涉及分子探针技术、生物样品快速分离技术、激光诱导荧光检测技术等相关领域。以目前典型的赤潮毒素（记忆缺失性贝毒、神经性贝毒、腹泻性贝毒、麻痹性贝毒）为研究对象，发挥荧光分子探针强选择性和高灵敏度的优势，简化样品预处理步骤，将微流控芯片快速分离技术与激光诱导荧光检测技术相结合，实现目标物从复杂混合提取液中快速、高效、高灵敏分离检测。在此基础上对相关海产品进行初步调研，分析和评估各类海产品赤潮毒素污染的风险性。本项目创新性的将微流控芯片快速分析技术应用到海洋赤潮毒素检测领域中，为海洋生物毒素现场诊断奠定理论基础，对保障食品安全具有重要的理论意义和应用价值。

中文关键词： 微流控芯片；赤潮毒素；快速；高灵敏；

英文摘要： In this project, a novel, fast and high-sensitivity analysis method for marine red tide toxins will be developed based on microfluidic chip. The project involves the molecular probe technique, biological sample fast separation technology, laser induced fluorescence detection technology and other correlation fields. In order to evaluate the feasibility of the analysis method, the typical red tides toxins (Amnesic shellfish poisoning, Neurotoxic shellfish poisoning, Diarrheic shellfish poisoning, Paralytic shellfish poisoning) at present will be used as the research object. Due to the fluorescent molecular probe has the advantages of high selectivity and sensitivity, the sample pretreatment will be simplified. By combining laser induced fluorescence detection technology, the microfluidic chip fast separation method for fast, high-efficiency and high-sensitivity detection of red tides toxins will be investigated. Based on the preliminary results，a trial will be designed to analyze and evaluate red tide toxins pollution in some seafood. In the project the microfluidic chip fast separation technology will be creatively applied on the marine red tide toxins research field. The analysis method will provide a theoretical basis for in-situ detecting red tide toxins. Meanwhile, it has great theoretical significance and ap

英文关键词： Microfluidic chip；Red tide toxins；Fast；High-sensitivity；