集成式红外加热荧光定量PCR微流控芯片及其在病原体基因检测中的应用研究

项目名称： 集成式红外加热荧光定量PCR微流控芯片及其在病原体基因检测中的应用研究

项目编号： No.21205131

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 分析化学

项目作者： 李博伟

作者单位： 中国科学院烟台海岸带研究所

项目金额： 25万元

中文摘要： 基于感染性病原体疾病是世界性的重要公共卫生问题之一，利用实时荧光定量PCR技术对病原体进行快速、灵敏、准确诊断和治疗的意义重大。但现有商品化荧光定量PCR仪器成本昂贵，体积庞大，操作复杂，不能满足当代社会对环境、食品安全和传染性疾病快速诊断的需求。微流控芯片作为新型的微分析技术平台，具有试剂消耗量低、结构微型化和功能集成化等优势。本项目拟采用最新的非接触式红外加热技术结合高效、低成本的LED诱导荧光检测技术，搭建"功能集成结构微缩"的实时荧光定量检测平台，实现常见病原体的DNA基因序列的实时荧光定量PCR检测。如蒙批准，本项目不但将建立一种崭新的具有普遍适用意义的微小型集成化的实时定量荧光PCR芯片平台，而且也为检测病原体提供一种快速、便携、高效且成本低廉的方法和手段。

中文关键词： 红外加热；非接触；微流控芯片；实时定量；PCR

英文摘要： Pathogen infections are one of the important public health problem worldwide, and the use of real-time quantitative PCR technique is rapid, sensitive, accurate diagnosis and treatment of significant pathogens of infectious diseases. Existing commercial quantitative PCR instrument is expensive, bulky and complicated to operate, that cannot meet the need for rapid diagnosis of contemporary society on the environment, food safety and infectious diseases. Lab-on-a-chip platform as a new type of micro-analysis, has the advantages of low reagent consumption, miniaturization and function integration. The project is proposed using the latest non-contact infrared heating technology combined with efficient, low-cost LED induced fluorescence detection technology, to build a "functional integration structure miniature" real-time fluorescence quantitative detection platform, and to achieve real-time fluorescence of the DNA gene sequences in common pathogens quantitative PCR detection.If approved, The project will not only establish a new universally applicable meaning miniaturized real-time quantitative fluorescent PCR chip platform, but also to provide a fast, portable, efficient and low cost methods for detection of pathogens.

英文关键词： Infrared heating；non-contact；microfluidic chip；real-time quantitative；PCR