江西及周边省份猪流行性腹泻病毒分子流行病学和致病机理的研究

项目名称： 江西及周边省份猪流行性腹泻病毒分子流行病学和致病机理的研究

项目编号： No.31260611

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 唐玉新

作者单位： 江西农业大学

项目金额： 50万元

中文摘要： 猪流行性腹泻（PED）是由PED病毒（PEDV）引起的一种高度传染性危害各年龄猪只的急性腹泻病，是重点防制的猪的传染病之一。近年来，PED在我国许多用灭活疫苗免疫接种的猪群中再度出现，并伴以高发病率（60%-100%）和仔猪的高死亡率（80%-100%）。导致此现象的原因，我们认为目前流行的PEDV野毒株在致病性、抗原性和免疫原性等方面发生了改变，使得现今使用的疫苗不能对PEDV野毒株感染提供有效免疫保护。为此，我们拟采用包括病毒宏基因组学技术在内的分子生物学技术和方法对江西和周边省份PEDV分子流行病学及发病机制进行研究:在全基因组水平上对疫苗株和流行野毒株进行分析比较，揭示它们的进化关系，确定它们的基因型和表型等重要分子生物学特征的异同；观察自然和人工感染猪的病理解剖及病理生理变化。为研发更有效和更安全的下一代PEDV疫苗、新的诊断工具和抗PEDV药物提供分子生物学和病理学科学依据。

中文关键词： 猪流行性腹泻；猪流行性腹泻病毒；宏基因组学；分子流行病学；发病机制

英文摘要： Porcine epidemic diarrhea (PED) is a highly contagious acute diarrheal disease caused by PED virus (PEDV), a member of the family Coronaviridae. Killed PEDV vaccines are being used for the prevention and control of PED. However, PED has been reemerging in immunized swine accompanied by an increased disease severity in recent years. A recent outbreak of PED swept more than10 provinces in southern China in 2010-2011, characterized by an almost 100% morbidity and 80%-100% mortality among neonatal piglets, resulting in more than one million piglets death. To reveal the mechanisms responsible for the severe form PED disease, we propose to investigate the molecular epidemiology of reemerging PEDVs currently circulating in Jiangxi and surrounding Provinces and the pathogenesis of predominant Chinese wild-type PEDV strains. We hypothesize that Chinese field strains of PEDV have altered in terms of pathogenecity, antigenecity, and immunogenecity, which leads to currently used PEDV vaccines fail to provide solid protection against the infections of those viruses. Viral Metagenomics, other state-of-the-art and conventional approaches in the fields of Molecular Virology, Epidemiology, Immunology, Biology and Bioinformatics will be employed to identify and characterize genetic and phenotypic variations at the entire geno

英文关键词： Porcine Epidemic Diarrhea；Porcine Epidemic Diarrhea Virus；Metagenomics；Molecular Epidemiology；Pathogenesis