硫化氢在拟南芥应答重金属镉胁迫过程中的信号作用及其机制

项目名称： 硫化氢在拟南芥应答重金属镉胁迫过程中的信号作用及其机制

项目编号： No.31300236

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 刘志强

作者单位： 山西大学

项目金额： 23万元

中文摘要： 硫化氢已经成为继一氧化氮和一氧化碳之后的第三种气体信号分子，其在哺乳动物体内心血管等方面的功能的已经得到证实，但是在植物中，其作为信号分子的作用尚不明确。我们以拟南芥为材料的前期工作发现如下现象：生理浓度的硫化氢处理可以明显增强拟南芥对重金属镉胁迫的耐受性；生理浓度的硫化氢熏蒸和重金属镉胁迫一样，可诱发拟南芥气孔关闭；生理浓度的硫化氢处理在提高拟南芥对重金属镉胁迫的耐受能力过程中，上调了脱落酸(ABA)信号途径中多个重要标记基因表达水平。结合这些实验证据和已有文献报道，本研究运用分子生物学、生物化学、植物生理学等研究手段，研究气体信号分子硫化氢在植物应答重金属镉胁迫过程中的信号作用及其机制；解析K+离子通道在其信号途径中的作用；了解硫化氢信号途径与ABA信号途径在植物应答重金属胁迫这一非生物胁迫中的相互关系与整合方式。

中文关键词： 硫化氢；重金属；信号转导；拟南芥；谷子

英文摘要： Hydrogen sulfide, following the nitric oxide (NO) and carbon monoxide (CO), has been proposed as the third gasotransmitter, the function of which is significantly implicated in the cardiovascular in mammals as a signaling molecules, but not in plants. Our preparatory work demonstrated that physiological concentrations of hydrogen sulfide fumigation notably enhanced the tolerance of Arabidopsis against cadmium stress and induced the stomatal closure, which was the same as treatmented by Cd, and the expression levels of important marker genes involved in abscisic acid (ABA) signaling pathways were raised. According to the phenomenon above and experimental evidence reported in the literature, we started the task -" The role and mechanism of hydrogen sulfide as a gasotransmitter in Cadmium stress response in Arabidopsis",and molecular biology, biochemistry and plant physiology were usd to resolve two questions: the function of K + ion channel in hydrogen sulfide signaling pathways, and the mutual relations and integration of hydrogen sulfide and ABA signaling pathways in plants response to the heavy metals stress.

英文关键词： hydrogen sulfide；heavy metal；signal transduction；arabidopsis；foxtail mille