项目名称: 新疆拜城油鸡肌内脂肪代谢相关基因mRNA表达的发育性变化研究
项目编号: No.31460584
项目类型: 地区科学基金项目
立项/批准年度: 2015
项目学科: 农业科学
项目作者: 沙尔山别克·阿不地力大
作者单位: 新疆农业大学
项目金额: 50万元
中文摘要: 拜城油鸡是我区优良的地方品种。该鸡种全身皮下脂肪分布均匀,在禽类中较为特别,油鸡故此得名。我国目前尚存的油鸡品种也只有北京油鸡和拜城油鸡两种。肌内脂肪(IMF)被认为是对肉品质有着极为重要的一项指标,找出影响IMF含量的主效基因或候选基因及其调控,是很多研究者的工作目标。本研究以影响肉品质的重要指标之一的IMF含量为突破口,从机体脂肪代谢有关的A-FABP、H-FABP、LPL、PPAR-γ、UCP3和Apo基因mRNA的表达量出发,用索氏抽提法和Real Time PCR法,测定和探讨拜城油鸡不同生长阶段IMF沉积、IMF沉积相关的上述基因表达的发育性变化以及与IMF含量的关联性,并筛选影响肌内鸡脂肪代谢的主效基因或候选基因,为今后的新疆拜城油鸡肉质性状的早期辅助选择并加快育种进程提供一定的科学依据,更为我区的珍惜遗传资源的保护与开发利用指出可行的方向。
中文关键词: 拜城油鸡;肉品质;肌内脂肪;基因表达
英文摘要: The intramuscular fat of muscle is supposed to be meat quality has a very important a index, find out the fat content of influence muscle lord effect genes or candidate genes and its regulation, is many researchers working goal. The research on the basis of previous studies, to worship in Baicheng-You chicken of Xinjiang and AA chicken as the research object, and explore the quality chicken different growth stages intramuscular fat of muscle and fat deposits intramusculat fat metabolism related gene(A-FABP 、H-FABP、PPAR-γ、LPL、UCP3 and Apo) expression of the development of the candidate of change, and these gene expression and muscle in the relevance of the intramuscular fat content, and influence the fat metabolism were muscle of the effect the genes; On this basis, muscle quality and muscle fat metabolism in related gene expression of mRNA effects for future chicken meat marker assisted selection and characters of the molecular level of quality control of chicken provide certain theoretical basis, more of our country cherish conservation of genetic resources development and utilization of providing some reference.
英文关键词: Baicheng-You Chicken;Meat Quality;IMF;Gene Expression