西瓜MYB转录因子对枯萎病抗性调控机理研究

项目名称： 西瓜MYB转录因子对枯萎病抗性调控机理研究

项目编号： No.31201632

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 吕桂云

作者单位： 河北农业大学

项目金额： 23万元

中文摘要： 前期研究中,我们利用抑制差减文库和芯片技术分析西瓜抗枯萎病反应的基因表达谱,筛选出一个参与西瓜抗性早期反应的转录因子ClMYB, QRT-PCR实验表明该基因在西瓜的抗病品种中优势表达，而感病品种中没有差异表达。MYB转录因子是指含有MYB结构域的一类转录因子，它参与次生代谢,应答激素的刺激，抵御外界胁迫和病原菌的侵染。本项目拟在前期研究和西瓜基因组数据基础上克隆该基因,进行分子生物学鉴定；利用Gateway技术构建正、反义表达载体进行转基因的功能验证；通过染色质免疫沉淀-深度测序Chip-seq技术鉴定目的转录因子的下游靶基因及调控的代谢途径；以明确转录因子ClMYB基因与西瓜抗枯萎病反应的关系及其调控抗性反应响应的途径。研究结果为深入解析该基因调控西瓜抗病性应答反应的分子机理提供了理论基础，为西瓜枯萎病的抗病育种和病害防治提供可靠的科学依据。

中文关键词： 西瓜；枯萎病；MYB转录因子；调控机理；

英文摘要： In a recent study , a novel MYB transcription factors, designated as ClMYB ,was isolated from watermelon root infected by Fusarium oxysporum f.sp. niveum using an Agilent custom microarray and suppression subtractive hybridization (SSH). The ClMYB genes were also induced during the incompatible interaction, but were repressed in the compatible interaction by Quantitative Real-Time PCR. In plant, MYB transcription factors play various roles in response to biotic and abiotic stimuli, as well as in secondary metabolism. The objective of this study is to clone ClMYB transcription factor on the basis of preliminary studies, and validate gene function by construction and transformation of the sense and anti-sense expression vector of ClMYB gene using gateway technology, and identify the downstream target genes and regulation of metabolic pathway of ClMYB gene by Chip-seq. These would reveal regulatory mechanism of ClMYB transcription factor gene in watermelon plants in response to Fusarium oxysporum f.sp. niveum infection. The studies also provide new insights into the understanding of the molecular mechanism in response of watermelon plants to Fusarium oxysporum f.sp. niveum infection and will be helpful in molecular breeding of Fusarium wilt resistant varieties and the development of rational strategies for control

英文关键词： watermelon；Fusarium wilt；MYB transcription factor；regulatory　mechanism；