空间辐射和微重力影响dauer期线虫DNA损伤修复的分子机制

项目名称： 空间辐射和微重力影响dauer期线虫DNA损伤修复的分子机制

项目编号： No.31270903

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 孙野青

作者单位： 大连海事大学

项目金额： 80万元

中文摘要： 为研究空间辐射的生物学效应，我们利用神舟八号飞船研究dauer期线虫的空间生物学效应，发现空间辐射和微重力影响了野生型线虫DNA损伤修复相关基因的表达，而dys-1突变株线虫的基因却无明显变化，提示了dys-1基因突变可能影响线虫对抗逆相关的基因表达。同时发现野生型和dys-1突变株线虫都有受空间辐射和微重力影响而特异变化的microRNAs (miRNAs)，而dys-1缺失可能特异性影响某些miRNAs的表达。本项目拟以这些变化的基因为切入点，全面分析和验证参与线虫DNA损伤修复调控的基因及对应的miRNAs；分析dys-1基因突变是否影响这些DNA损伤修复相关基因的表达，哪些miRNAs参与其中；研究空间辐射和微重力是否影响不同的miRNAs及其调控的基因网络，是否会交互影响；同时全面分析相关的蛋白质表达情况，揭示空间辐射和微重力环境调控DNA损伤修复的分子机制。

中文关键词： 空间辐射；微重力；DNA损伤响应；线虫；dys-1突变

英文摘要： To understand biological effect of space radiation, we studied biological effects of C. elegans at dauer stage taken by Shenzhou 8 spacecraft to space. Our results show that space radiation and microgravity affect the expression of genes related with DNA damage repair in wild-type (WT) of C. elegans, but not dys-1 mutant, indicating that deficiency of dys-1 might affect the gene expression of stress resistance. Moreover, space radiation and microgravity specifically affect the expression of microRNAs (miRNAs) in both WT and dys-1 mutant of C. elegans, and some miRNAs might be specifically affected by deficiency of dys-1 gene. In this project, we will regard these altered genes as the entry point to do a comprehensive analysis, identify the genes involved in DNA damage repair and miRNAs in C. elegans, analyze whether deficiency of dys-1 affects expression of these genes related with DNA damage repair, in which how many miRNAs are involved and which miRNAs are regulated. We will study whether space radiation and microgravity affect the expression of different miRNAs and gene network regulated by miRNAs, and examine whether some interaction exists in the gene network. In addition, we will also do a comprehensive analysis of proteins. Overall, our study will reveal the molecular mechanism about DNA damage repair reg

英文关键词： space radiation；microgravity；DNA damage response；C. elegans；dys-1 mutation