解偶联蛋白2对早期断奶仔猪肠道活性氧调控和肠道发育的作用及其分子机制解析

项目名称： 解偶联蛋白2对早期断奶仔猪肠道活性氧调控和肠道发育的作用及其分子机制解析

项目编号： No.31201806

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 畜牧学与草地科学、兽医学、水产学

项目作者： 陈小连

作者单位： 江西省农业科学院

项目金额： 23万元

中文摘要： 早期断奶应激一直是困扰养猪业的一个难题，也是动物营养学界亟待解决的命题之一。针对本课题组前期研究结果表明，断奶后仔猪体内产生大量活性氧，并与肠黏膜结构和功能呈显著负相关，解偶联蛋白2（UCP2）具有调控活性氧产生和能量代谢的功能，而有关UCP2如何调控断奶仔猪肠道活性氧产生及与肠道发育的关系目前并不明确。本研究应用免疫组化、荧光定量RT-PCR、Western-blot、基因重组和RNA干扰等技术，分析断奶前后仔猪肠道UCP2的分布和表达规律及其与活性氧和肠道发育的关系，研究调控UCP2基因表达对应激状态下猪肠上皮细胞增殖、凋亡与能量代谢的作用，深入探讨UCP2对仔猪肠道活性氧调控和肠道发育的关系及其分子机制，研究成果对通过调控UCP2表达途径，研发新型功能性饲料维持肠道健康以缓解仔猪断奶应激具有重要的指导意义，丰富断奶应激理论的内涵，为今后研究动物生理和病理损伤机制提供科学依据。

中文关键词： 解偶联蛋白2；活性氧；肠道发育；早期断奶；仔猪

英文摘要： Early weaning stress of piglets is always a tough problem in pig industry and animal nutriology. Our previous work has shown that reactive oxygen species (ROS) in the piglets at 3 days post weaning was significantly increased and negatively correlated with intestinal structure and function. Uncoupling protein 2 (UCP2), a member of the mitochondrial inner membrane carrier family, is implicated in the control of ROS production and energy metabolism. There have not been related reports on UCP2 expression pattern in the intestine of post weaning piglets and effects on intestinal development and ROS production in weaning piglets up to now. This project aims to explore the distribution and expression pattern of UCP2 in the small intestine of weaning piglets using immunohistochemistry, real-time RT-PCR and Western-blot. Construct recombinant plasmid containing over-expressed UCP2 gene or siRNA mediated silencing of the UCP2 gene, and transfect to the intestinal porcine epithelial cell. In order to further discussion of the molecular mechanisms of UCP2 on the intestinal development from tissue, molecule and cell level, the effect of different UCP2 gene expression level (over expression, normal expression, silence and suppression expression of UCP2 gene) on proliferation, apoptosis and energy metabolism in the damaged in

英文关键词： Uncoupling protein 2；Reactive oxygen species；Intestinal development；Early weaning；Piglets