复苏植物牛耳草脱水胁迫下内质网应激反应的调控机制

项目名称： 复苏植物牛耳草脱水胁迫下内质网应激反应的调控机制

项目编号： No.31300230

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 王波

作者单位： 中国科学院植物研究所

项目金额： 23万元

中文摘要： 内质网质量控制(ER-QC)是维护内质网基质中蛋白的合成和转运动态平衡的重要机制。细胞受胁迫时，ER-QC平衡机制被打破引发内质网应激反应(ER stress response)。但过度的内质网应激反应会导致细胞凋亡。复苏植物牛耳草在干旱锻炼后叶片获得耐干性，细胞严重脱水时虽然发生内质网应激，但仍可存活并复苏，其中机制尚未见报道。针对此科学问题，本项目拟进行三方面研究：①脱水程度与内质网应激及细胞凋亡发生的时间和程度之间的对应关系；②干旱锻炼前后的牛耳草叶片中，内质网应激诱导剂处理对耐脱水性和细胞凋亡发生程度及相关基因表达的影响；③内质网应激信号途径和ER-QC的关键成分在干旱锻炼前后的牛耳草脱水胁迫下的表达调控与功能及作用机制。本研究首次关注复苏植物在脱水胁迫下细胞内蛋白折叠转运的动态平衡重建的机理，将从控制内质网应激反应、抑制细胞凋亡的角度扩展和加深对复苏植物耐干复苏机制的理解。

中文关键词： 牛耳草；耐脱水性；质量控制；BhbZIP60；BhHSP70-1

英文摘要： The endoplasmic reticulum quality control (ER-QC) is an important mechanism for maintaining homeostasis in the endoplasmic reticulum (ER). Under stress, breaking down of homeostasis in the ER trigger the ER stress response. Excessive ER stress response will lead to apoptosis. Resurrection plant Boea hygrometrica can tolerant desiccation after drought acclimation.ER stress occurs during dehydration, however,apoptosis is inhibited so that the cells Boea hygrometrica can survive and resurrected after rehydration. In order to understand the regulatory mechanism of ER stress pathway in Boea hygrometrica,three issues will be addressed in this project: ① The correlation between the course of water deprivation and the extent of ER stress and cell apoptosis, ② the effect of ER stress inducer on desiccation tolerance and apoptosis in fresh and drought acclimated Boea hygrometrica leaves，and ③ function and regulation mechanism of key components of the ER stress signaling pathways and the ER-QC in fresh and drought acclimated Boea hygrometrica. The results would lay the foundation for the research on reestablishment of protein homeostasis, and expand and deepen our understanding on the mechanisms of desiccation tolerance of resurrection plants from the aspect of regulation of ER stress and inhibition of ER stress induced ce

英文关键词： Boea hygrometrica；desiccation tolerance；QC；BhbZIP60；BhHSP70-1