砧木调控嫁接黄瓜果实表面蜡粉形成的硅代谢机制

项目名称： 砧木调控嫁接黄瓜果实表面蜡粉形成的硅代谢机制

项目编号： No.31272211

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 魏珉

作者单位： 山东农业大学

项目金额： 80万元

中文摘要： 嫁接是提高设施栽培黄瓜生物和非生物逆境抗性，提高产量的重要农艺措施，但不同砧木对嫁接黄瓜果实表面蜡粉形成的影响明显不同，嫁接组合体的硅代谢特性是主要原因。本项目选择具有不同去蜡粉能力的砧木，研究嫁接和自根黄瓜硅吸收分配特性与果实表面蜡粉形成的关系，借助透射、扫描电子显微镜（TEM、SEM）技术观察蜡粉形成过程及其超微结构特征；克隆不同类型砧木根系中的硅转运蛋白相关基因Lsi1和Lsi2，利用实时荧光定量PCR（qRT-PCR）技术检测不同砧穗组合和不同环境条件下的表达特性；通过高通量基因表达谱分析技术，构建黄瓜果实中控制蜡粉形成的差异基因表达谱，确定候选基因，并研究其表达特性；以期从分子水平上揭示砧木影响嫁接黄瓜果实表面蜡粉形成的机理。研究结果将为黄瓜优质栽培和砧木育种提供理论指导。

中文关键词： 黄瓜；蜡粉；砧木；硅；硅转运蛋白

英文摘要： Grafting is one of important agricultural techniques to improve resistance to biotic and abiotic stresses, and to increase production of protected cucumber,but the significantly different impact was found in bloom formation on fruit surface of cucumber grafted on different rootstocks,in which the silicon metabolism in grafted plants plays a major role. Rootstocks with different de-blooming ability will be choosed in the project to study the relationship between silicon absorption and allocation and bloom formation on fruit surface of cucumber.The ultrastructure and formation process of bloom will be observed with TEM and SEM. The genes Lsi1 and Lsi2 for silicon transporter protein will be cloned from roots of different types of rootstocks, and their expression activities will be also detected by using qRT-PCR under different environmental conditions.The gene expression profile controlling bloom formation in cucumber fruit will be constructed by using high-throughput gene expression profile technology, and followed by identification of candidate genes and detection of their expression characteristics. The purpose of the project is to reveal the mechanism of rootstocks affecting bloom formation on fruit surface of grafted cucumber at the molecular level, and the research results will provide a theoretical guidance

英文关键词： Cucumber；Rootstock；Bloom；Silicon；Silicon transporters