项目名称: 吡氟禾草灵对菊科杂草的选择性作用机制
项目编号: No.31272066
项目类型: 面上项目
立项/批准年度: 2013
项目学科: 农业科学
项目作者: 罗小勇
作者单位: 青岛农业大学
项目金额: 80万元
中文摘要: 在众多的菊科杂草中,刺苞菊属植物硬毛刺苞菊对禾本科杂草专用除草剂吡氟禾草灵具有特殊的敏感性,初步研究发现该敏感性的形成与药剂快速诱导活性氧的产生和导致膜结构的破坏有关,这与该除草剂在禾本科植物上的作用机制截然不同。本项目进一步围绕吡氟禾草灵在菊科植物间的选择性作用机制问题,采用形态学、细胞学、生物化学、放射线同位素标记及仪器分析方法,通过研究和比较该药剂与对照药剂吡氟氯禾灵在硬毛刺苞菊(对吡氟氯禾灵不敏感)及三叶鬼针草(菊科,对吡氟禾草灵和吡氟氯禾灵均不敏感)植物体内的代谢途径、诱导的活性氧种类、对顶端分生组织的作用以及与干扰植物生长素之间的联系,明确其除草作用是否由不同的代谢途径所引起,所诱导的膜脂质过氧化及膜结构破坏是否与干扰植物生长素的作用有关,进而阐明其诱导活性氧形成的途径以及在不同敏感性菊科杂草间的选择性作用机制,以期为该药剂的有效利用和新型除草剂的创新提供科学依据。
中文关键词: 吡氟禾草灵;硬毛刺苞菊;活性氧;IAA氧化;IAA氧化酶
英文摘要: Among many composite weed species, Acanthospermum hispidum, an Acanthospermum family plant, is specially susceptible to fluazifop-butyl, a graminicide. According to the preliminary studies, we discovered that the mechanism of fluazifop-butyl in A. hispidum may be related to its action for induction of reactive oxygen species (ROS) and membrane disruption. This is distinctly different from its action in grass weeds. Around the scientific problem of selective action mechanism of fluazifop-butyl in compositae plants, through the studies and comparison each other of metabolic pathways, induced reactive oxygen species, action on apical meristem and the relationship between herbicide action and the role of plant auxin interference of fluazifop-butyl and haloxyfop-ethyl in Acanthospermum hispidum and Bedens pilosa by the methods of morphology, cytology, biochemistry, radioactive isotopes mark and instrumental analysis. The following problems are expected to be clarified: whether exist the specifity target site in A. hispidum for fluazifop-butyl, whether the action of fluazifop-butyl in A. hispidum is induced by the different metabolism, whether the herbicide-induced membrane lipid peroxidation and membrane structure destruction related to plant auxin interference, and to further expound the pathway of ROS formation and
英文关键词: Fluazifop-butyl;Acanthospermum hispidum;Reactive oxygens;IAA oxidation;IAA oxidases