稀土掺杂LiLuF4@LiGdF4核壳纳米晶的制备及多模生物标记应用

项目名称： 稀土掺杂LiLuF4@LiGdF4核壳纳米晶的制备及多模生物标记应用

项目编号： No.61505077

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 无线电电子学、电信技术

项目作者： 鞠强

作者单位： 南京工业大学

项目金额： 20万元

中文摘要： 多模生物标记材料，由于其具有实现特定靶向、无损伤、高空间分辨率、实时以及三维扫描多模式成像能力，而被认为是新一代生物标记材料，然而，目前关于多模生物标记材料的研究仍非常有限。本项目致力于研发同时具有光学成像、磁共振成像（MRI）和X-射线断层扫描成像（CT）功能的稀土掺杂LiLuF4@LiGdF4核壳纳米标记材料，开展LiLuF4@LiGdF4的合成、表征、表面修饰、生物分子偶联和多模生物成像，探索这一类多模纳米材料的造影能力与颗粒尺寸、核壳结构以及表面官能团的关系，从中研制出一种高效多模纳米标记材料，通过光学成像、MRI和CT等多模成像，实现对神经肌无力症临床诊断标记物SMN1的超敏检测。预期本项目研制的多模纳米标记材料在发光强度以及MRI和CT造影能力方面均表现出优于荧光染料、稀土螯合物以及碘制剂等的良好性能，在疾病的早期检测以及疾病发展机理研究等领域发挥重要作用。

中文关键词： 稀土上转换纳米荧光颗粒；无机纳米粒子；磁性纳米探针

英文摘要： Multimodal bioprobes, owing to their ability to realize target specific, noninvasiveness, high spatial resolution, 3-D tomography, and real-time bioimaging, have been regarded as a new and promising generation of bioprobes. However, the research on multimodal bioprobes is still in its infancy. In this project, we aim to synthesize lanthanide-doped LiLuF4@LiGdF4 core-shell bioprobes, which could simultaneously realize the optical bioimaging, MRI and CT. The synthesis, characterization, surface modification, bio-conjugation and multimodal bioimaging will be systematically studied. The relationship between the capability of bioprobe and the size of nanoparticle, core-shell structure and surface functionalized groups will be investigated in an effort to improve the bioimaging effect. To this regard, we will develop one efficient and practical nanoprobe applied for early detection of SMN1 (clinical marker in diagnostic of neuromuscular disease) by in vivo optical, MRI, CT multiomodal imaging. Such multimodal LiLuF4@LiGdF4 core-shell bioprobes are expected to show more superior feature in the optical intensity, and contrast ability than commercial contrast agents such as organic fluorophores, lanthanide-chelate, and iodinated products, and thus may have great potentials in the field of early disease detection and investigation of disease mechanism.

英文关键词： Upconversion nanoparticle;Nanoparticle;Magnetic nanolabel