飞秒激光激励的氧化石墨烯多光子荧光效应及其在活体功能成像中的应用研究

项目名称： 飞秒激光激励的氧化石墨烯多光子荧光效应及其在活体功能成像中的应用研究

项目编号： No.61275190

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 无线电电子学、电信技术

项目作者： 钱骏

作者单位： 浙江大学

项目金额： 78万元

中文摘要： 氧化石墨烯纳米颗粒的生物成像应用是近期国际上研究的热点，而基于多光子荧光效应的生物显微成像具有成像深度大，空间分辨率高，生物损伤小等诸多优点，将受激发射荧光机理引入多光子荧光成像，可进一步提高信号强度和对比度。在本项目中，我们将利用飞秒激光器、激光共聚焦扫描显微镜构建多光子自发/受激发射多光子荧光显微系统，并将其用于氧化石墨烯纳米颗粒的自发/受激多光子荧光效应的研究。我们还将建立小鼠脑组织、斑马鱼等活体实验模型，实现氧化石墨烯对特定结构、器官（如脑部神经元，斑马鱼血管等）的标记，并利用自发/受激发射多光子荧光显微系统对其进行活体成像，揭示具体的生物行为和机理。

中文关键词： 氧化石墨烯；纳米颗粒；多光子荧光显微术；脑组织；斑马鱼

英文摘要： The application of graphene oxide in bio-imaging is a hot spot of recent academic research. Microscope imaging based on multiphoton induced fluorescence has lots of advantages, such as large imaging depth, high spatial resolution, low damage towards bio-samples.The signal intensity and contrast of multiphoton fluorescence microscopy could further be enhanced by introducing stimulated emission mechanics. In this project, we plan to build a spontaneous/stimulated emission multiphoton fluorescence microscope system by utilizing a femtosecond laser and a confocal scanning microscope, and use it to investigate the multiphoton fluorescence effect of graphene oxide nanoparticles. We will also setup animal models (e.g., brain tissue of mice, zebrafish) in our lab for in vivo experiment. We will use graphene oxide to target certain structures and organs of the animal models (e.g., neurons of mice, blood vessel of zebrafish), and perform in vivo bio-imaigng by utilizing the spontaneous/stimulated emission multiphoton fluorescence microscope, to illustrate detailed biological avtivities and mechanics.

英文关键词： graphene oxide；nanoparticles；multiphoton fluorescence microscopy；brain tissue；zebrafish