渍涝胁迫下玉米体内多胺变化及其分子调控机制

项目名称： 渍涝胁迫下玉米体内多胺变化及其分子调控机制

项目编号： No.31201162

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 王群

作者单位： 河南农业大学

项目金额： 23万元

中文摘要： 多胺是植物体内重要的调节物质，在植物抵御逆境胁迫及信号转导中承担着重要角色，前期研究初步发现外源多胺能有效缓解玉米的渍涝胁迫伤害，但渍涝胁迫下玉米体内多胺的变化规律及其响应胁迫的生理和分子机制尚不清楚。本研究以耐涝性不同的玉米幼苗为材料，利用HPLC技术和RT-PCR技术检测渍涝胁迫下玉米幼苗体内多胺组分、游离态多胺含量和主要膜蛋白上结合态多胺含量变化及其功能；明确渍涝胁迫下多胺合成酶和氧化酶活性变化及其基因差异表达。阐明多胺在玉米响应渍涝胁迫中的生理作用及分子机制，以丰富多胺信号转导和多胺抗性生理研究，为研发作物渍涝灾害缓解技术提供理论依据。

中文关键词： 玉米；渍涝；多胺；多胺合成酶活性；基因表达

英文摘要： Polyamines play an important role in the regulation of senescence and stress-resistance of plants. In our experment results showed that exogenous polyamines could alleviated the harm to maize roots and leaves by waterlogging. But little is known about the effect of waterlogging stress on PAs content, metabolism and molecular mechanisms of maize. In the present study, Four maize cultivar seedlings with differentwaterloging-resistances were used to study the relationship between PA changes and waterlogging -resistance. Free polyamines content in the seedling roots and non-covalent bound polyamines on plasma membrane, mitochondrial membrane and ribosome will be tested by HPLC. Activity of polyamines biosynthesis and oxidase will be invested by spectrophotometry and gene expression of polyamines biosynthesis and oxidase will be quantified by RT-PCR. The aim of this study was to explore: ①polyamines components and free polyamines contents in maize seedlings under waterlogging; ②the content and function of the bound polyamines in the membrane protein; ③the relationship between the activity and gene expression of polyamines biosynthesis and oxidase and the treatment of exogenous polyamines. The result of this project would do help to elucidate the contents variation and function of polyamines under waterlogging stress

英文关键词： maize；waterlogging stress；polyamine；polyamine biosynthesis；genes expression