功能化磁性纳米介质萃取新技术及其在痕量大环内酯类抗生素检测中的应用研究

项目名称： 功能化磁性纳米介质萃取新技术及其在痕量大环内酯类抗生素检测中的应用研究

项目编号： No.21477037

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 化学工业

项目作者： 章飞芳

作者单位： 华东理工大学

项目金额： 80万元

中文摘要： 旨在发展复杂样品中痕量大环内酯类抗生素（Macrolide antibiotics,MALs）检测的功能化磁性纳米介质萃取新技术及其分析方法。基本策略为：构建以磁性纳米颗粒为内核、表面功能化涂层为壳的新型核壳型磁性纳米介质；拟发展功能化硅胶涂层和表面分子印迹聚合物(Surface molecular imprinting polymer,SMIP)涂层实现对复杂基体中目标物的高选择性萃取。功能化硅胶涂层：根据MALs分子弱碱型脂溶性强的特点，拟通过发展限制进入型内孔表面键合C18或发展硅胶涂层的表面键合方法，引入极性C18混合官能团（比如阳离子交换基团-C18）以增强萃取效果和改善纳米介质的分散性；SMIP涂层：分别以红霉素、阿奇霉素和麦迪霉素为模板分子在磁性纳米颗粒表面构建相应的SMIP涂层实现对常见MALs高选择性萃取。通过上述萃取技术、结合高效液相色谱发展痕量MALs快速检测技术。

中文关键词： 磁性纳米材料；限制进入材料；功能化硅胶涂层；表面分子印迹；大环内酯类抗生素

英文摘要： The project is aimmed to develop functionalized magnetic nanoparticles-based extration technologies and its application for extraction and determination of trace macrolide antibiotics (MALs) in food.The basic strategis are as follows. Novel core-shell magnetic nanoparticles will be fabricated by introducing funcationalized coating onto magnetic nanoparticles as core. Highly selective coatings including funcationalized silica coating and surface molecular imprinting polymer (SMIP) coating will be developed onto magnetic nanoparticles for selective extraction of target MALs from very complex matrix. For functionalized silica coating, restricted access material (RAM) with chemically-bonded C18 groups inside the inner pores and the chemical bonding methodologies will be investigated for introducing mixed funcational groups (e.g. C18 and cation exchange groups) in consideration of weak base and hydrophobic property of MALs compounds, aimming to enhance extraction ability and improve the dispersity of nanoparticles. For SMIP coating, take erythrocin, azithromycin and medemycin as module molecule, the corresponding SMIP coating onto magnetic nanoparticles will be respectively fabricated for selective extraction of common used MALs. By combination of above extraction technologies and high performance liquid chromatography, it is aimmed to devlope fast extraction and determination technology of trace MALs.

英文关键词： functionalized magnetic nanoparicle;restricted access material;functionalized silica coating;surface molecular imprinting;macrolide antibiotics