拟穴青蟹促性腺激素释放激素（GnRH）生殖旁分泌作用及信号通路分析

项目名称： 拟穴青蟹促性腺激素释放激素（GnRH）生殖旁分泌作用及信号通路分析

项目编号： No.41476119

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 天文学、地球科学

项目作者： 叶海辉

作者单位： 厦门大学

项目金额： 89万元

中文摘要： 本项目开展拟穴青蟹GnRH 生殖旁分泌作用和信号通路分析，主要内容：（1） GnRH 的生殖旁分泌作用，主要观察GnRH 对卵母细胞生发泡破裂（GVBD）、成熟促进因子（MPF） 激活、钙离子电流变化、GnRH 受体表达的影响。（2）GnRH 信号通路分析，研究卵母细胞cAMP 信号通路，MAPK 信号通路和磷脂酰肌醇信号通路三者与MPF 激活的关系，并确认接受GnRH 调控的信号通路。本项目率先开展了甲壳动物GnRH 旁分泌作用的研究，开辟了甲壳动物内分泌学研究的一个新领域；首次分析了甲壳动物卵母细胞信号通路与MPF 激活的关系，并对接受GnRH 调控的信号通路进行了鉴定。本项目以卵母细胞为研究材料，利用膜片钳技术等手段分析卵母细胞成熟的机制，突破了甲壳动物没有细胞系的平台限制，相关研究结果可为甲壳动物诱导产卵的技术创新提供科学依据。

中文关键词： 拟穴青蟹；GnRH；旁分泌；卵母细胞；信号通路

英文摘要： This project plans to investigate the reproductive paracrine of gonadtropin releasing hormone (GnRH) and its signaling pathways in the mud crab, Scylla paramamosain, including following topics: ⑴ The reproductive paracrine function of GnRH, by observing GnRH's effect on germinal vesicle breakdown (GVBD), the activation of maturation promoting factor (MPF), the change of Ca2+ current, and the induction of the GnRH-receptor expression; ⑵ The analysis of GnRH signaling pathways, by studying the relationship between cAMP, MAPK and phosphatidylinositol signaling pathways and the activation of MPF, and confirm whether they accept the regulation of GnRH. This project takes a lead in studying the paracrine function of GnRH in crustacean, thus develops a new domain in crustacean endocrinology. It is for the first time that the relationship between oocytes signaling pathways and activation of MPF is analyzed, and signaling pathways which accept the regulation of GnRH is identified. This project will break through the limits of lacking cell lines in crustacean in that oocytes are taken as experimental materials, and patch clamp techniques were used to study the maturation mechnism of oocyte. The results would provide new scientific proofs for spawning induction in crustacean. In brief, this project has great theoretical importance and potent application value.

英文关键词： Scylla paramamosain;GnRH;paracrine;oocyte;signaling pathway