UVB启动人角质形成细胞NF-κB信号通路的microRNA调控机制

项目名称： UVB启动人角质形成细胞NF-κB信号通路的microRNA调控机制

项目编号： No.81502743

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 杨玲

作者单位： 中国人民解放军联勤保障部队第九二〇医院

项目金额： 18万元

中文摘要： 已有研究表明，中波紫外线（UVB）引起细胞核因子κB（NF-κB）信号通路的激活是光敏性皮肤病的重要发病机制，近年陆续发现UVB可引起细胞多种微RNAs（micro RNAs）的表达改变。micro RNAs与靶基因间存在着广泛的相互调控，并与细胞分化和疾病发生密切相关。基于此，本课题拟采用micro RNA芯片技术研究UVB辐照人角质形成细胞的micro RNA表达改变，用反义寡核苷酸、基因转导、RNA干扰等技术分析各相关micro RNA对NF-κB信号通路中IκB或IKK基因的调控。通过上述研究，旨在完善UVB辐照角质形成细胞的micro RNAs表达谱，初步探讨UVB启动人角质形成细胞NF-κB信号通路的microRNA调控机制，这对于深入揭示光敏性皮肤病局部的炎症信号通路调控，以及未来开展探索靶向性的干预措施或疾病治疗方法的研究具有重要意义。

中文关键词： 中波紫外线；角质形成细胞；核因子-κB；；微RNA

英文摘要： It’s demonstrated that mid-wave ultraviolet (UVB) activate NF-κB signal pathway, which is an important pathogenesis of photosensitive dermatoses. Recent researches reveal changes of many micro RNAs expressions in UVB-irradiated cells. Mutual regulations, between micro RNAs and target genes, are involved in diverse processions such as cell differentiation or developments of many diseases. So in this study, micro-RNAs expressions in UVB-irradiated keratinocytes would be analyzed by micro-RNA array firstly. Then, the regulating mechanisms of some special micro-RNAs on IκB or IKK, key genes in NF-κB signal pathway, would be explored by antisense oligonucleotide, gene transfection and RNA interference. Based on these results, micro RNAs spectrum and their functions in NF-κB signal pathway would be further investigated in UVB-irradiated keratinocytes. The above-mentioned achievements might play an important role in deeply revealing inflammatory signal pathways in local photosensitive dermatoses as well as provide new directions or targets for the intervention and treatment of photosensitive dermatoses.

英文关键词： mid-wave ultraviolet (UVB);keratinocyte;nuclear factor-κB (NF-κB);micro-RNA