拟南芥C3H14和C3H15基因调控次生细胞壁形成的分子机制研究

项目名称： 拟南芥C3H14和C3H15基因调控次生细胞壁形成的分子机制研究

项目编号： No.31300165

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 柴国华

作者单位： 中国科学院青岛生物能源与过程研究所

项目金额： 23万元

中文摘要： 植物次生细胞壁不仅在水分、营养的长距离运输和机械支撑中起重要作用，而且也是纤维生物质储存的主要场所，因而对次生细胞壁形成机制的研究具有重要意义。在模式植物拟南芥中，次生细胞壁的形成受多个基因家族的调控，形成一个复杂的调控网络，但目前其分子调控机制，特别是转录后调控机制知之甚少。我们前期研究表明，拟南芥两个CCCH锌指蛋白基因C3H14和C3H15均在茎和花中大量表达，编码的蛋白具有RNA结合活性，冗余调控次生细胞壁的形成，且分别在茎秆和花药次生细胞壁中起主导作用。以此为基础，本项目拟通过免疫定位、RIP-Chip、REMSA等技术，进一步深入分析C3H14和C3H15基因在次生细胞壁形成中的生物学功能，鉴定其参与细胞壁形成的下游各自作用靶标，揭示其在转录后水平上调控次生细胞壁形成的分子机制，为未来利用基因工程手段调控植物次生细胞壁的形成奠定基础。

中文关键词： 次生细胞壁；C3H14/15；RNA 结合蛋白；调控机制；

英文摘要： Plants have evolved to posses secondary cell walls that not only provide mechanical support for their growing body and serve as a conduit for long-distance transport of water and solutes，but also contain the main compoments,partically in trees.Therfore，it's important to understand and modify secondary cell wall regulatory mechanism.In model plant Arabidopsis, secondary cell wall formation is coordinatedly regulated by multiple gene families and forms a complex network.However,it is not clear for its secondary cell wall regulatory mechanism, specially at the posttranscriptional level to date. Recently, we functionally characterized two CCCH zinc finger proteins C3H14 and C3H15 and found that the two genes were highly expressed in stems and flowers, their proteins were able to bind to Ribohomopolymers and redudantly regulated secondary cell wall formation with predominantly in stems for C3H14 gene and anthers for C3H15 gene. Based on the aboved results, this project aims to futher analyze the biological functions of C3H14 and C3H15 genes, use RIP-Chip and REMSA to identify downstream target genes involved in cell wall formation, respectively, and finally clarify the molecular regulatory mechanism of C3H14 and C3H15 in secondary cell wall formation at the posttranscriptional level. The results in this project may p

英文关键词： Secondary cell wall；C3H14/15；RNA binding protien；Regulatory mechanism；