猕猴桃维生素C高效合成与积累的分子机理研究

项目名称： 猕猴桃维生素C高效合成与积累的分子机理研究

项目编号： No.31471157

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 刘永胜

作者单位： 合肥工业大学

项目金额： 85万元

中文摘要： 猕猴桃因富含维生素C（Vc）而受到广泛关注。已经完成的中华猕猴桃（Actinidia chinensis）代表品种红阳的基因组和转录组数据揭示猕猴桃基因组中与Vc合成代谢相关基因发生了显著的数量倍增，但其转录活性、生化与生物学功能还不清楚。本项研究将利用组学和正、反向遗传学、生化与分子生物学以及转基因技术系统探讨基因培增、转录时空调控、限速酶活性、Vc代谢中间产物消长与终产物的积累以及氧化态和还原态（Vc）循环再利用等影响猕猴桃Vc高效合成和积累的分子调控机理，鉴定分离新的Vc合成代谢和积累的重要功能基因，为园艺植物品质改良和分子设计育种提供新的技术途径。

中文关键词： 基因功能分析；遗传学；基因表达调控；分子育种；功能基因组学

英文摘要： Kiwifruit (Actinidia) is an economically and nutritionally important fruit crop with remarkably high content of ascorbic acid (Vitamin C, Vc). Although marked difference in Vc contents between Actinidia species as well as between interspecific cultivars of Actinidia chinensis was found, the molecular mechanisms of Vc hyperaccumulation in the fruit of Actinidia are unknown and have been paid great attention. Based on the tremendous datasets of the genome sequencing and RNA-seq transcriptome of Hongyang, a widely cultivated variety derived from Actinidia chinensis, we plan to further investigate the potential molecular mechanisms of Vc hypersynthesis and hyperaccumulation in kiwifruit. Multiple approaches and cutting-edge techniques including comparative genomics, transcriptomics, forward and reverse genetics, biochemical and molecular methods as well as transgenic technology will be extensively adopted in our study to elucidate effects of Vc-associated gene duplication, gene transcriptional capacity, rate-limiting enzyme activities, as well as its metabolic intermediates and its associated redox enzymes on the hyperaccumulation of Vc. Hence, our aim is to identify the key structural genes and/or regulatory genes which could be used to enhance Vc accumulation not only in the fruit of Actinidia but also in other crops and/or vegetables via molecular breeding and genetic modification.

英文关键词： Gene functional charaterization;Genetics;Gene expression and regulation;Molecular breeding;Functional genomics