动物源性食品中青霉素残留的电化学核酸适配体传感检测机制研究

项目名称： 动物源性食品中青霉素残留的电化学核酸适配体传感检测机制研究

项目编号： No.31501563

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 李芳

作者单位： 河南科技大学

项目金额： 19万元

中文摘要： 青霉素类抗生素广泛应用于畜禽疾病的预防和治疗，是动物源性食品中常见的抗生素残留物。目前，抗生素残留已成为制约我国动物源性食品安全的首要因素之一，研究青霉素类抗生素残留的痕量检测技术具有重要的理论和现实意义。核酸适配体具有高亲和力、特异性，易合成、易保藏等特点，有望取代抗体或弥补抗体不足成为新一代生物识别分子应用于生物传感检测领域。本课题拟选取阿莫西林和氨苄西林为研究对象，采用切换-荧光磁珠SELEX技术筛选核酸适配体，分析结构特征，并利用光谱法和PCR法研究其与抗生素之间的相互作用机理，测定解离常数。在此基础上，结合纳米技术和信号放大技术，构建高特异性的、稳定的、可反复测定的、多通路、痕量青霉素类抗生素核酸适配体传感检测方法，为抗生素残留的痕量检测和适配体传感测定研究提供一个新思路。

中文关键词： 核酸适配体；；青霉素残留；电分析；动物源性食品；SELEX

英文摘要： Penicillins, which is the typical antibiotic residues in foods of animal origin, has been extensively used in animal husbandry and poultry production to prevent and treat bacterial infections. At present, antibiotic residues has become one of the primary inhibiting factor in animal-derived food safety. It is important to develop the trace analysis technology for the detection of penicillins residues in animal-source food. Aptamer, which can be easily produced via chemical synthesis and has the superior thermal and chemical stability, the high affinity and specificity for their targets, has been considered as an alternative recognition elements for the development of biosensors and bioassays. In this research, the amoxicillin (AMO) and ampicillin(AMP) specific aptamers will be selected by a modified SELEX method, which is an extended form of Toggled-SELEX combined with Flu-Mag SELEX by employing target and counter target molecules sequentially different to generate specific AMP and AMO aptamers. The interactions between targets and aptamers will be examined by spectrometry and PCR. Based on the aptamer - target affinity, a novel stable, high specific and reusable electrochemical aptasensor for the trace analysis of penicillins residues in animal-derived food would be developed with the nanotechnology and signal amplification.

英文关键词： aptamer;penicillin residue;electrochemical analysis;animal-source food ;SELEX