项目名称: 识别霉菌毒素的催化活性纳米材料辅助核酸适配体传感器阵列研究
项目编号: No.21305158
项目类型: 青年科学基金项目
立项/批准年度: 2014
项目学科: 数理科学和化学
项目作者: 文芳
作者单位: 中国农业科学院北京畜牧兽医研究所
项目金额: 25万元
中文摘要: 食品及饲料中霉菌毒素的快速、灵敏且准确的检测是保障其安全的重要前提。现有的霉菌毒素快速检测方法存在成本高、灵敏度较差、检测霉菌毒素种类有限、操作繁琐等问题,急需发展高灵敏、低成本、可实现多种霉菌毒素快速检测的方法。本课题提出一种催化活性纳米材料辅助的核酸适配体传感器阵列,用于食品及饲料中多种霉菌毒素的识别检测。利用一系列具有不同长度不同序列的核酸适配体作为识别单元,具有催化活性的纳米材料作为信号转导单元,通过耦合适配体的分子识别功能与催化纳米材料的高效信号转换和放大功能,提高传感器的选择性和灵敏度。通过核酸适配体可控调节纳米材料的催化活性,建立识别反应与催化活性之间的相关关系,得到不同霉菌毒素的特征指纹图谱,结合模式识别,实现对多种霉菌毒素的准确、高灵敏检测。本项目为解决高特异性霉菌毒素适配体种类有限和筛选困难的问题提供了一种新思路,有望成为霉菌毒素分析的另一种新兴的检测手段。
中文关键词: 核酸适配体;传感器;霉菌毒素;RT-qPCR;荧光
英文摘要: apid, sensitive and accurate detection of mycotoxins is an important prerequisite to safeguard the security of food and feedstuffs. The existing methods for mycotoxins detection and monitoring suffer from the problem of high cost, poor sensitivity, complicated operation and limited targets. Therefore, it is urgent to develop an ultra-sensitive, cost-effective, and high throughput method for rapid detection of multiple mycotoxins. The project has proposed a novel sensor array based on catalytically active nanomaterials assisted aptamers for recognition and detection of mycotoxins in food. A series of aptamers with different length and sequence will be employed as recognition units, while nanomaterials featuring intrinsic catalytical activity will be ultilized as signal transduction units and signal amplifiers in the sensor array. The sensitivity and discrimination ability of the sensor array would be greatly improved by coupling the selectivity of aptamers and the catalytic ability of the nanomaterials. The interaction between aptamers and mycotoxins will be systematically investigated to screen a series of aptamers, which have moderated binding affinities with different mycotoxins. The catalytic activity of the nanomaterials can be effectively modulated by aptamers through changing the aggregation state of the n
英文关键词: Aptamer;Sensor;Mycotoxin;RT-qPCR;Fluorescent