血管平滑肌细胞表型相关lncRNA的筛查及其在转录调控中与核内microRNA的互作效应

项目名称： 血管平滑肌细胞表型相关lncRNA的筛查及其在转录调控中与核内microRNA的互作效应

项目编号： No.81200215

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学一处

项目作者： 孙绍光

作者单位： 河北医科大学

项目金额： 23万元

中文摘要： 在人类和哺乳动物细胞中，长链非编码RNA (lncRNA)在基因转录调控中发挥着非常重要的作用，但迄今为止，与心血管相关lncRNA的研究还刚刚起步。关于microRNA (miRNA)调控机制的研究目前主要集中在胞质中miRNA对mRNA的转录后调控，而有关核内miRNA参与的转录调控研究还非常少见。对于核内miRNA能否与lncRNA产生互作及其在转录调控中的分子效应还完全未知。本项目拟以血管平滑肌细胞（VSMC）作为模型，首先，分析不同表型VSMC中的lncRNA表达谱，鉴定与VSMC表型密切相关的核内lncRNA，揭示其参与转录调控的分子机制；然后，重点探讨核内miRNA与lncRNA相互作用，阐明这种互作对lncRNA与其他转录因子互作的影响，以及在VSMC表型相关的转录调控中的分子效应。这对于深入研究非编码RNA互作在转录调控中的效应及其在VSMC表型转化的功能具有重大意义。

中文关键词： 长链非编码RNA；microRNA；Hand2；血管平滑肌细胞；

英文摘要： In human and mammalian cells, the long non-coding RNAs (lncRNAs) play very important roles in gene transcriptional regulation, but so far, the study of cardiovascular lncRNAs has just started. The major researches of present about the regulation mechanism of microRNAs (miRNAs) just concern on the post-transcriptional regulation of miRNAs-mRNAs interactions in the cytoplasm, and the study about mechanism of nuclear miRNAs involved in transcriptional regulation is very rare. Whether the nuclear miRNAs interact with lncRNAs to involve in transcriptional regulation is still completely unknown. This project intends to use vascular smooth muscle cell (VSMC) as a model. Firstly, analyze lncRNA expression profiling in different phenotype VSMC, identify several nuclear lncRNAs which play important regulatory roles in VSMC phenotype transformation, and clarify the molecular mechanisms of transcriptional regulation; and then, explore the interaction between lncRNAs and nuclear miRNAs, and identify the impact of this interaction on the other interaction between lncRNAs and transcription factors, and clarify its molecular effect in the transcriptional regulation of the VSMC phenotype transformation. It is great significant to reveal the effect of non-coding RNAs interaction in the transcriptional regulation and the function

英文关键词： lncRNA；microRNA；Hand2；VSMC；