lnc-CENPQ-2在颞叶内侧型癫痫发病机制中的作用

项目名称： lnc-CENPQ-2在颞叶内侧型癫痫发病机制中的作用

项目编号： No.81671281

项目类型： 面上项目

立项/批准年度： 2017

项目学科： 医药、卫生

项目作者： 崔志强

作者单位： 中国人民解放军总医院

项目金额： 25万元

中文摘要： 颞叶内侧型癫痫（mTLE）是最常见的一种癫痫类型，海马硬化为其主要发病原因，多种分子、信号通路参与了癫痫的发生发展。长链非编码RNA（lncRNA）作为一种重要的调控因子，在神经系统中参与了神经细胞的增殖、分化、轴突的形成、重塑等一系列过程，然而在癫痫发病机制方面的研究鲜有报道。本课题前期基因芯片结果显示mTLE硬化的海马与正常海马组织中有497个IncRNAs具有表达差异性，294个高表达，203个低表达，靶标预测结果显示lnc-CENPQ-2及靶基因CPLX3高度相关，因此选择两者作为研究对象，拟用qRT-PCR、细胞转染、基因敲除、Westernblot、免疫组化等方法对其功能进行体内、体外实验验证，从而明确 lnc-CENPQ-2及CPLX3在mTLE发病机制中的作用。本课题从lncRNA这一新的角度对mTLE的发病机制进行研究，为癫痫的防治提供了重要的理论依据。

中文关键词： 颞叶内侧癫痫；海马硬化；长链非编码RNA；；lnc-CENPQ-2

英文摘要： Mesial temporal lobe epilepsy (mTLE) is one of the most common types of epilepsy. Hippocampal sclerosis is the main cause of the disease, and varieties of molecules and signaling pathways are involved in the development of epilepsy. The long non-coding RNA (lncRNA) as an important regulatory factor involved in nerve cell proliferation, differentiation, axonal formation and remodeling in the nervous system, however, few lncRNA research has been reported in epilepsy. Early results of this study showed there were 497 IncRNAs with different gene expression in the tissues of hippocampal sclerosis and normal hippocampus, 294 high expression, 203 low expression. The target gene prediction analysis showed that there was highly correlated between lnc-CENPQ-2 and target gene CPLX3, so both of them were choosen as the research subjects. In order to clarify the role of lnc-CENPQ-2 and cplx3 in the pathogenesis of MTLE, many methods are used in vivo and in vitro experiments, like qRT-PCR, cell transfection, gene knock-out, Western blot and immunohisto-chemistry. In conclusion, from a new perspective of lncRNA, this study discusses its role in the pathogenesis of mTLE, which provides an important theoretical value in the prevention and treatment of epilepsy.

英文关键词： mesial temporal lobe epilepsy;hippocampal sclerosis;long non-coding RNA; lnc-CENPQ-2