茶树响应低温胁迫的microRNA发掘及其调控机制研究

项目名称： 茶树响应低温胁迫的microRNA发掘及其调控机制研究

项目编号： No.31270729

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 李叶云

作者单位： 安徽农业大学

项目金额： 82万元

中文摘要： 冻害是我国大部分茶区茶树优质高产栽培中重要限制因子，随着全球气候异常，近年表现尤为突出。茶树抗寒分子机制主要集中在功能基因发掘与表达特征方面的研究，而有关转录后调控研究甚少。本项目以耐寒茶树品种为材料，基于独有的茶树基因组数据，应用转录组深度测序、小RNA Solexa深度测序、miRNA芯片杂交、基因表达谱芯片和荧光定量PCR技术，并结合生物信息学手段，发掘与鉴定响应低温胁迫的茶树miRNAs，进行过表达分析，并采用降解组测序鉴定靶向基因。研究ABA、脱水和低温三种处理下miRNAs及靶向基因的表达差异与相互关系，比较品种间miRNA及靶向基因表达模式差异。这将有助于明确茶树中抗寒相关miRNAs及其靶基因功能与表达模式，在转录后水平上揭示出茶树响应低温胁迫的调控机制，为茶树抗寒品种选育、抗逆基因发掘提供新的理论支持，也对进一步认识植物抗逆miRNAs的功能具有重要意义。

中文关键词： 茶树；miRNA；低温胁迫；表达谱；靶基因

英文摘要： Cold stress is an important limiting factor of tea plant cultivation for high yield and high quality in Chinese most tea-growing areas, which are particularly obvious with global climate deteriorating in recent years. The studies on molecular mechanism of tea plant cold-tolerance mainly focused on the screening and expression characteristics of functional genes but there were a few works in regulation of post-transcription. In this project, on the base of tea plant genome data, combined with bioinformatics, transcriptome deep sequencing, small RNA Solexa deep sequencing, miRNA microarray hybridization, gene expression profile and quantitative real-time PCR technique were utilized to screen and identify miRNAs responsive to low-temperature stress as well as study their functions through transgenic tobacco over expression with cold-resistant tea varieties as materials. Differential expression and interrelation of miRNAs and targeted genes were investigated under ABA, dehydration and low-temperature treatments whilst expression pattern differentia of miRNAs and targeted genes were compared in tea varieties, which was helpful for elucidating the function and expression characteristics of miRNAs and targeted genes related to cold-tolerance of tea plant and indicating the regulation mechanism responsive to low-tempera

英文关键词： Camellia sinensis；miRNA；cold stress；expression profiling；target gene