量子点生物效应的热化学研究

项目名称： 量子点生物效应的热化学研究

项目编号： No.20873096

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 刘义

作者单位： 武汉大学

项目金额： 39万元

中文摘要： 量子点有许多独特荧光性能，在生命科学中有很好的应用前景，国际上受到广泛关注，其生物效应研究处于起步阶段。本项目将宏观和微观研究手段相结合，以CdSe/ZnS和CdTe量子点为主要对象，采用微量热、显微、光谱、生化分析和分子生物学等技术，结合热动力学方法，在细胞、细胞器、生物大分子等层次上，从结构和功能上，研究了不同量子点的生物效应及其机制。建立了量子点对生命代谢影响的热动力学模型，获取量子点对生命代谢动态过程影响的热动力学性质；深入认识了不同量子点对生命代谢动态过程的影响途径及其作用机制。在不同层次、不同水平上，揭示了量子点生物效应的作用机制，及量子点结构与热动力学参数的关系，为量子点的高效合成、应用及生物安全性评价提供理论依据，促进不同学科在高水平、深层次上的交叉。 该项目的相关研究工作共发表SCI源刊论文15篇，其中影响因子>5.0的4篇、3.0-5.0的6篇。先后获得Bioorganic & Medicinal Chemistry Most Cited Article 2003-2010 Award (Elsevier)、湖北省优秀博士学位论文。该项目较好的完成了研究任务。

中文关键词： 量子点；生物效应；热化学；热动力学；机制

英文摘要： Quantum Dots have special fluorescence properties, it has a lot of applications in life sciences, but the biological effect and toxicity are still unknown. In this project, CdSe/ZnS and CdTe quantum dots were selected as the main system, and lot of research techniques, such as microcalorimetry, microscopy, spectroscopy etc. have been combined. With the help of thermokinetics, the biological effect and mechanism of different quantum dots were investigated from structure and function on the level of cell, cell organelle and biomacromolecular. The thermokinetic model of the efect of quantum dots on living metabolism was established, and a lot of thermokinetic properties were obtained. The biological effect mechanism of different quantum dots, and the relationship between quantum dots structure and thermokinetic parameters, were disclosed. The research provided a lot of important information on synthesis, application and biosafty evaluation of quantum dots. The research result of NSFC 20873096 has published 15 SCI papers. It has formed a new way for interdisplinary and infiltration among chemistry and biology. The research of this project has won 2 awards, such as "Bioorganic & Medicinal Chemistry Most Cited Article 2003-2010 Award (Elsevier)", "Excellent Ph.D. Degree Thesis of Hubei Province". The research of NSFC 20873096 has reached its anticipation.

英文关键词： QDs; biological effect; thermochemistry; thermokinetics; mechanism