月季切花失水胁迫MYB类转录因子分离及功能鉴定

项目名称： 月季切花失水胁迫MYB类转录因子分离及功能鉴定

项目编号： No.31501798

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 姜新强

作者单位： 青岛农业大学

项目金额： 20万元

中文摘要： 月季是全球第一大切花。已经明确，失水胁迫是导致月季切花采后流通损耗的主要影响因素，极易造成花朵畸形、开放异常，严重影响观赏和使用价值。但是，在月季花瓣失水胁迫过程中的转录调节机理尚不明确。筛选和鉴定参与花瓣失水胁迫的重要转录因子便成为解析转录调节机理的一个重要课题。申请者前期已通过抑制性差减杂交和芯片技术，从花瓣失水胁迫文库中筛选到52个RhMYBs参与花瓣失水胁迫进程，初选出6个与花瓣失水胁迫关系密切的RhMYBs。本课题拟对参与失水胁迫的关键RhMYBs进行分离和鉴定，通过过表达拟南芥和月季体内的基因沉默技术对候选基因的功能进行鉴定；开展RhMYBs的生化特性和转录活性分析；通过酵母单杂交技术筛选出RhMYBs的上游基因，确定其转录调控机制。本项目将从转录水平上阐明RhMYBs参与切花月季失水胁迫耐性的转录调节机制，为月季的抗逆分子育种及遗传改良提供坚实的理论基础和基因资源。

中文关键词： 月季；水分胁迫；RhMYBs；转录调控；基因功能

英文摘要： Rose (Rosa hybrida) is one of the most important ornamental plants worldwide. It is known that water dehydration is the main limiting factor that terminates the ornamental value of the flower, resulting in flower wilts and abnormal flower opening. However, the molecular mechanisms underlying the dehydration tolerance are still unclear. It is important to investigate the candidate transcription factor genes and their biological functions involved in response to dehydration tolerance in cut rose flowers. With the help of suppression subtractive hybridization and cDNA microarray methods, 52 MYB type transcription factors were found in rose flower dehydration library, and six RhMYBs were closely related to dehydration tolerance in rose petals. We want to isolated and analyzed the key RhMYBs in rose petals under dehydration conditions. In addition, the gene silencing, transforming to model plants and functional complementation methods were also used to investigate the candidate genes biological function. Moreover, expression analysis, transactivation and yeast-one hybrid analysis were also used to investigate the transcription regulaton of RhMYBs. Therefore, our study will uncover the physiology and molecular mechanism of the RhMYBs regulatory network in the dehydration tolerance in rose petals at the transcription level. These analysis of RhMYBs will provide a rational scientific and practice basis for the utilization of them and enrich the rose plant species. Moreover, the selected genes are informative for the genetic crop improvement by non-conventional methods.

英文关键词： Cut rose;Dehydration;RhMYBs;Transcription regulaton;Gene function