G-蛋白Rab3A与突触结合蛋白I的相互作用及其对突触递质释放的影响

项目名称： G-蛋白Rab3A与突触结合蛋白I的相互作用及其对突触递质释放的影响

项目编号： No.31271135

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 王贤纯

作者单位： 湖南师范大学

项目金额： 80万元

中文摘要： 大脑是信息处理中心以及各种生理活动和行为的控制中心。突触是大脑神经元之间发生功能联系的部位，许多与突触功能有关的生理生化过程，如神经递质释放与激活、神经递质受体的调节以及信号传导级联放大等都是由突触中构成复杂网络的突触蛋白相互协调完成的。因此，深入开展突触蛋白质及其相互作用研究有助于加深对突触结构与功能的了解。据文献报道，G-蛋白Rab3和突触结合蛋白Synaptotagmin 是调节突触囊泡释放的两种具有相反作用的关键蛋白质。我们的前期工作证明，Rab3A能通过与Synaptotagmin I 的直接相互作用，影响突触膜的融合。本项目研究将在前期工作的基础上，利用蛋白质组学及相关技术研究哺乳动物大脑突触中Rab3A与Synaptotagmin I 的相互作用机理及其在突触递质释放调节中的作用，从而进一步加深对突触传递分子机制的了解。

中文关键词： G-蛋白Rab3A；突触结合蛋白；相互作用；分子机制；递质释放

英文摘要： The brain is the center for integrating information and controlling various physiological activities and behaviors. Synapses are the sites for functional connection of the neurons in brain. Many physiological and biochemical processes related to the functions of synapses, such as transmitter release and activation, transmitter receptor modulation，signal transduction cascades and so on, are all performed by the intricate network of synaptic proteins cooperatively. Therefore, a deep investigation into the synaptic proteins and their interactions is helpful to further understand the struture and function of the synapses.It has been reported that Rab3 and synaptotagmin are two critical proteins with oposite functions in the regulation of synaptic vesicle exocytosis. Our previous work demonstrated that Rab3A directly interacts with synaptotagming I and therefore affects the synaptic membrane fusion. The present project will, based on the previous work,investigate the mechnisiam of Rab3A interaction with synaptotagmin I and its roles in the regulation of synaptic transmitter release in the mammal brain synapaes using proteomic and related techniques, so as to further deepen the understanding of the molecular mechnism of synaptic transmission.

英文关键词： G-protein Rab3A；Synaptotagmin；Interaction；Molecular mechanism；Transmitter release