低营养低剪切条件下厌氧颗粒污泥形成的群感机理及调控

项目名称： 低营养低剪切条件下厌氧颗粒污泥形成的群感机理及调控

项目编号： No.51208464

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 建筑环境与结构工程学科

项目作者： 冯华军

作者单位： 浙江工商大学

项目金额： 25万元

中文摘要： 针对课题组开发的填料式折流板反应器（CABR）在低营养低剪切条件下可实现厌氧污泥颗粒化的现象，本项目拟从群体感应（Quorum Sensing, QS）角度探查厌氧颗粒污泥的形成机理。在建立厌氧颗粒污泥体系信号分子富集和检测方法的基础上，通过设置对照组，识别低营养低剪切条件下厌氧污泥颗粒化过程中关键信号分子，结合颗粒污泥的形态学特征、物理特性、化学组成以及微生物种群的时空分布，探明环境压力-信号分子-污泥颗粒化之间的内在关系；通过投加关键信号分子以及关键信号分子的抑制剂或降解酶，验证信号分子对厌氧颗粒污泥的诱导作用机理及强化调控策略的可行性，这有助于我们更好的理解和阐明低营养低剪切条件下厌氧颗粒污泥的形成机理。项目的实施对于提高厌氧反应器高效性和稳定性，提升我国在废水生物处理的基础理论和工艺领域的研究水平将具有十分重要的意义。

中文关键词： 厌氧颗粒污泥；信号分子；群体感应；胞外聚合物；

英文摘要： Anaerobic sludge granulation was observed in carrier anaerobic baffled reactor (CABR) at low nutrition and low shear. The mechanisms of anaerobic sludge granulation are investigated with quorum sensing theory. Based on the solution to extraction and detection of signal molecules in anaerobic granular sludge reactor, key signal molecules, which contribute to anaerobic sludge granulation, are identified through setting control. Besides, the inner relationships among external stress, signal molecular secretion and sludge granulation are ascertained, through reviewing morphology, physical property, chemical constitution and microorganism space-time distribution characteristics of anaerobic granular sludge. Induction of signal molecular for anaerobic sludge granulation is confirmed through dosing the key molecular signals and quorum sensing inhibitors/signal degradation enzymes. At the same time, the feasibility of regulation strategy with quorum sensing for anaerobic sludge granulation is affirmed. The study is benefit for us to understand and proclaim the mechanisms of anaerobic sludge granulation at low nutrition and low shear. Moreover, it is of important significance to carry out the project for enhancing efficiency and stability of anaerobic bioreactor, and improve the foundation theory and technology in our wa

英文关键词： anaerobic granular sludge；signal molecules；quorum sensing；extracellular polymer substances；