白血病细胞微观力学性能表征

项目名称： 白血病细胞微观力学性能表征

项目编号： No.11202139

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 唐斌

作者单位： 南方科技大学

项目金额： 25万元

中文摘要： 白血病细胞力学特性与白血病的临床治疗密切相关：白血病细胞的硬度及其与细胞基质之间的粘附力影响白血病淤滞症发病率，同时白血病细胞与造血干细胞力学特性之间的差异有可能提供一种新的体外净化白血病的方法。白血病细胞力学的相关研究现仍处于起步阶段，由于测量方法上的缺陷，现有数据有很大的离散型，白血病细胞力学特性在疾病中的具体作用因此仍不很清楚。课题组在近年提出了率跳跃模型用以修正机械力学测试中可能存在的滞弹性效应，提高测试的准确度；同时也发明了了光钳压痕技术，并对白血病细胞力学性能进行了初步的测试。在此项研究中，我们将应用率跳跃模型改良现有的光钳压痕技术，并将其应用于对白血病细胞力学系统研究，从而准确表征白血病细胞在不同条件下的力学特性，研究细胞周期，药物处理等对白血病细胞特性的影响，并探讨其在白血病临床治疗中的作用。

中文关键词： 白血病；压痕测试；细胞力学；弹性模量；光钳

英文摘要： The biomechanical properties of leukemia cells are closely related to the clinical treatment of leukemia: the deformability of leukemia cells and the adhesion force between the cells and their excellular matrix are two major factors in determining the risk of leukostasis, which is a key reason for the death of patient suffered leukemia. The difference between the biomechanical properties of leukemia cells and Hematopoietic Stem Cells will possibly provide a new technique to purify the bone marrow used for autotransplant. However, the investigation of biomechanics of leukemia cells is still at this early stage, only a few studies have been done. Moreover, due to the limit of current micro/nano mechanical characterization techniques, e.g. the existence of viscoelastic effects during the measurement, the mechanical properties of leukemia cells reported so far have a large scattering and are not very reliable. Recently, we purposed a rate-jump method to address the viscoelastic effects which can be applied to various mechanical testing platforms, and we also invented a new technique to performing indentation test on cells using optical trap. In this study, we will further modify the optical trap indentation technique by apply rate-jump method for correcting the viscoelastic effect during the measurement. The modifie

英文关键词： leukemia；indentation；cell mechanics；elastic modulus；optical tweezers