组织工程关节软骨再生修复作用的在体无创监测方法研究

项目名称： 组织工程关节软骨再生修复作用的在体无创监测方法研究

项目编号： No.30870639

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 杨柳

作者单位： 中国人民解放军第三军医大学

项目金额： 34万元

中文摘要： 组织工程技术为关节软骨缺损再生修复的新治疗策略。作为组织修复执行者的种子细胞的在体迁徙分布、增殖分化及转归过程，目前尚无安全无创、实时动态的监测手段。新近的磁共振成像（MRI）在体示踪磁标记细胞技术为其提供了新思路。本项目在前期完成纳米超顺磁性氧化铁（n-SPIO）标记骨髓间充质干细胞（MSCs）MRI 示踪方法研究的基础上，拟进行新型可控降解性n-SPIO 的研制、体外n-SPIO 标记种子细胞（骨髓MSCs、软骨细胞）适宜方法、磁标记物种子细胞生物相容性评价、MR 成像在体示踪磁标记种子细胞的技术方法研究，并以绿色荧光蛋白细胞示踪技术为对照，完成其组织工程技术修复关节软骨缺损动物实验应用研究，以及SPIO 标记物生物相容性与生物安全性评价。以此，为组织工程关节软骨种子细胞的在体示踪提供一种安全无创、实时动态的新技术和新方法，并为组织工程关节软骨产品临床应用的即时监测与疗效评定奠定基础。

中文关键词： 超顺磁性氧化铁；聚乳酸-聚醚共聚物；磁共振成像；种子细 胞；关节软骨组织工程

英文摘要： Tissue-engineering therapies hold promise for the treatment of articular cartilagedefects to achieve the regeneration to hyaline cartilage. However, there is still a lack of understanding regarding the characteristics of the seeded cells in repairing defects.The development of tissue-engineering therapies requires an efficient and noninvasive technique to monitor the in vivo behavior of implanted cells in host tissue and thus help understand the characteristics of the seeded cells.Cellular MR imaging is a rapidly growing field that aims to visualize targeted cells labelled by SPIO nanoparticles in living organisms. The aim of this study is to coat iron oxide nanoparticles with poly-DL-lactide-polyethylene glycol（PELA）to form superparamagnetic particles, investigate the influence of magnetic labeling on the biological properties of the seeded cells, evaluate in vivo magnetic resonance imaging with 1.5T system tracking for the surviva1,migration and differentiation of magnetically labeled MSCs in animal cartilage defect model, and determine the feasibility of MR imaging detect magnetically labeled seeded cells for articular cartilage tissue engineering noninvasively and repeatedly, the seeded cells labelled with green fluorescent protein (GFP) used as the control. Thus develop an efficient and noninvasive imaging technique to track seed cells will help understand cell functions, determine the efficacy of cell therapies, and optimize therapeutic protocols to increase the likehood of cartilage regeneration.

英文关键词： Supermagnetic iron oxide particles (SPIO); Poly-DL-lactide-polyethylene glycol（PELA）; Magnetic resonance imaging(MRI); Seeded cell; Articular cartilag