水生克隆植物的表型可塑性及表观遗传学机制

项目名称： 水生克隆植物的表型可塑性及表观遗传学机制

项目编号： No.31470451

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生态学

项目作者： 高雷

作者单位： 华南师范大学

项目金额： 80万元

中文摘要： 克隆植物的表型可塑性一直是生态学研究的重要方向之一。然而其发生及调节的分子遗传学机制却鲜有研究。本课题以水生入侵克隆植物凤眼莲为研究对象，试图研究其在不同水深环境下的表型可塑性及产生表型可塑性的DNA甲基化基础。研究内容主要包括：温室条件下，不同水深梯度培养凤眼莲，研究凤眼莲母株及子株在形态、生物量、及光合生理、养分含量等方面的差异；同时野外调查不同水体凤眼莲的分布特征、分株形态特征，养分含量等。试图研究克隆植物凤眼莲的表型可塑性及适应环境的生态学意义。同时，对温室实验的凤眼莲母株和子株进行取样，通过DNA甲基化敏感扩增多态性技术（MSAP），研究母株及子株DNA甲基化水平在不同水深梯度下的差异，试图探讨克隆植物表型可塑性的表观遗传学调节机制。该研究将从分子学角度进一步揭示克隆植物对异质环境的适应性调节机制。

中文关键词： 克隆植物；表型可塑性；表观遗传学；DNA甲基化；生物入侵

英文摘要： The phenotypic plasticity of clonal plants has been one of the important directions of ecology research. However, the molecular genetics mechanism of phenotypic plasticity was rarely studied. An aquatic invasive clonal plant Eichhornia crassipes will be studied in this project, and we try to study the phenotypic plasticity and its DNA methylation foundation of Eichhornia crassipes in different depths of the water environment. The research includes: under greenhouse conditions, Eichhornia crassipes is cultivated in water with different depths, and was studied the differences between ramets in morphology, biomass, and photosynthesis, nutrient concentration; the distribution of Eichhornia crassipes of water bodies was also studied. The aim is to study the phenotypic plasticity and ecological significance of adaptive of Eichhornia crassipes. At the same time, the parent and daughter ramets of Eichhornia crassipes are sampling in greenhouse experiment, , the difference of DNA methylation pattern of parent and daughter ramets in different water depth gradient will be studied through the DNA methylation-sensitive amplified polymorphism ( MSAP ), we try to explore the epigenetic regulation mechanism of phenotypic plasticity of clonal plant. The study will further reveal the adaptability regulation mechanism of clonal plant in heterogeneous environment from epigenetic angle

英文关键词： Clonal plant;Phenotypic plasticity;Epigenetic;DNA mythlation;Biological invasion