菊花CmWRKY51参与分枝调控的分子机理

项目名称： 菊花CmWRKY51参与分枝调控的分子机理

项目编号： No.31501792

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 宋爱萍

作者单位： 南京农业大学

项目金额： 23万元

中文摘要： 菊花是我国十大传统名花和世界四大切花之一，观赏和经济价值极高。分枝是切花菊最重要的园艺性状之一，决定其栽培方式、生产成本与观赏品质。目前，菊花分枝的生理机理与基因工程改良已有比较深入研究，但分枝调控的分子机制仍不明确。我们前期研究发现菊花CmWRKY51在调控植物分枝中发挥着重要作用，本项目拟通过亚细胞定位、表达模式分析及利用超表达和融合抑制技术获得CmWRKY51转基因菊花，明确CmWRKY51基本功能；克隆CmWRKY51下游直接调控基因及其启动子，分析CmWRKY51的结合元件并验证，明确CmWRKY51的转录调控机制；通过酵母双杂交筛选CmWRKY51的互作蛋白，并运用BiFC和GST Pull-down进行体内体外验证，揭示CmWRKY51的翻译后调控机制，最终阐明CmWRKY51参与菊花分枝调控的分子机制，为菊花分枝性状的分子设计育种提供理论基础和优异基因储备。

中文关键词： 菊花；器官形成；基因功能；基因调控；植物激素

英文摘要： Chrysanthemum is one of ten traditional famous flowers in China and a leading ornamental species, second only to the rose in terms of its market value. Branching is an important horticultural traits of chrysanthemum, which determined the cultural style, production costs, and ornamental quality. With the increasing cost of human resource, manual control on cut-flower chrysanthemum branching characteristics is becoming the main costs of production. In recent years, some progresses have been achieved in physiological, genetic and biotechnology research of chrysanthemum branching development, but the molecular mechanism is still not clear. Based on the previous research, CmWRKY51 gene was suggested as a critical regulator of plant branching. Through the subcellular localization, expression pattern analysis, and creating CmWRKY51 over-expression/ chimeric repressor gene-silencing transgenic chrysanthemum, basic functions of CmWRKY51 will be clarified. Cloning and analyzing the downstream genes and their promoters, which will clear the transcriptional regulatory networks of CmWRKY51. The yeast-two-hybrid assay will be applied to screen CmWRKY51 interacting proteins, then using the BiFC and GST Pull-down to validate both in vivo and in vitro. These evidences will reveal the post-translational regulation mechanism of CmWRKY51. Finally, we will clarify molecular mechanism of CmWRKY51 involved in the regulation of chrysanthemum branching. Our proposal can provide a theoretical basis and the elite gene pool for molecular assisted breeding of chrysanthemum branching traits.

英文关键词： Chrysanthemum;Organ formation;Gene function;Gene regulation;Plant hormone