白桦C2H2型锌指转录因子的耐盐调控机理研究

项目名称： 白桦C2H2型锌指转录因子的耐盐调控机理研究

项目编号： No.31470676

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 李莉

作者单位： 东北林业大学

项目金额： 85万元

中文摘要： 植物C2H2型锌指蛋白是成员众多的转录因子家族，在植物生长发育和非生物胁迫反应等过程中具有重要作用。我们在前期研究中鉴定了五个能被盐胁迫诱导表达的白桦C2H2型锌指蛋白基因（命名为BpZFP）, 研究证实其中的四个BpZFP基因可能参与白桦的耐盐应答。本项目在前期工作的基础上，拟对其中两个BpZFP 基因进一步开展以下的研究：（1）克隆BpZFP启动子；鉴定启动子中包含的重要耐盐相关顺式作用元件；研究其上游的调控因子和信号通路。（2）研究BpZFP所调控的下游靶基因；分析这些基因的产物所参与或调控的代谢途径。本项目的目的是研究BpZFP转录因子基因调控白桦耐盐的分子机理，为锌指蛋白基因在林木抗盐基因工程上的应用提供理论依据和优良基因。

中文关键词： 白桦；盐胁迫；C2H2型锌指转录因子；转录调控

英文摘要： The plant C2H2 zinc finger proteins represent a large of transcription factor family and numerous members of this family have been shown to play diverse roles in many biological processes, including plant growth, development and stress responses. In the previous study, five C2H2-type zinc finger transcription factor genes, named BpZFP, were identified that their expressions are induced by high-salt stress and four of them were confirmed that their involvement in salt tolerance in Birch. On these bases, we will further investigate two genes in two aspects: （1）To clone their promoters and define the cis-elements involved in the expression of BpZFP gene under high-salt stress and involved in signal transduction of stresses and analyze their upstream regulators and signal transduction pathway; （2）To identify the downstream target genes and the metabolic pathways regulated by these genes encoding products. This proposed project may contribute to reveal the molecular mechanism of the C2H2-type zinc finger transcription factor gene in regulation of salt stress tolerance in Birch and provide the theoretical and practical bases in genetic engineering to improve the stress tolerance in trees.

英文关键词： Betula platyphylla;Salt stress;C2H2-type zinc finger transcription factors;Transcriptional regulation