超声辐照载药纳米囊泡对宫颈癌靶向治疗及疗效监测的实验研究

项目名称： 超声辐照载药纳米囊泡对宫颈癌靶向治疗及疗效监测的实验研究

项目编号： No.81201178

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 影像医学与生物医学工程

项目作者： 张新玲

作者单位： 中山大学

项目金额： 23万元

中文摘要： 化疗是目前宫颈癌治疗不可或缺的辅助手段，但全身用药副作用大、疗效较差。采用超声微泡为载体有利于药物在病灶区域的聚集，提高治疗的靶向性。而应用纳米技术制备的纳米微泡具有粒径小、容易引入靶向基团及可通过血管内皮细胞屏障等优点，较目前的微米级微泡更易实现靶向治疗。本研究拟在前期已合成高分子材料聚合物纳米微泡的基础上，进一步制备具有高靶向性及超声控释和显像功能的纳米聚合物囊泡，同时负载化疗药紫杉醇及可液/气相变的五氟丁烷，携带抗人宫颈癌单克隆抗体单链片段（ScFv）进一步提高微泡的主动靶向传输，以裸鼠宫颈癌SiHa细胞皮下移植瘤为模型，采用低频超声辐照控制药物释放及吸收，并与气化产生的聚合物微泡协同作用，以达到靶向治疗及显像的双重目的。本研究的成功将为宫颈癌靶向治疗及监测提供一种全新的方法，对于提高宫颈癌化疗效果，降低化疗副作用、改善患者预后具有重要意义。

中文关键词： 宫颈癌；纳米颗粒；高强度聚焦超声；靶向治疗；疗效检测

英文摘要： Chemotherapy is an indispensable adjunctive method to cervical cancer. As conventional systemic medication often represents low effect and high rate of adverse incident, targeted-chemotherapy has gained great concern for its potential superiority. Usually used as a drug-carrier, ultrasonic microbubble can promote the aggregation of drug in the targeted lesion and enhance therapy effect. Compared with ultrasonic micron- bubble, Nano-microbubble (created by nanotechnology) seems even more suitable for targeted-chemotherapy. Nano-microbubble has very small size, which make it easily to get through vascular endothelial barrier and reach the targeted lesion. Nevertheless, targeting radicals can be easily introduced into the nano-microbubbles to improve their targeting capability. We have successfully developed nano-microbubbles using high polymer materials in earlier study. In present project, we plan to introduce ScFv and other specific radicals into the nano-bubbles. Thus these bubbles will possess high targeting ability and can be released by ultrasonic control. Paclitaxel and pentafluorobutanen will be loaded in the bubbles as chemotherapy agents. This specially designed compound will be tested in nude mouse model with cervical cancer and evaluate its effect, efficiency and adverse incidence. If successful, thi

英文关键词： cervical cancer；nanoparticle；High Intensity Focused Ultrasound；targeted therapy；therapeutic effect evaluation