人造细菌鞭毛基于DPPC脂质体的表面功能化研究

项目名称： 人造细菌鞭毛基于DPPC脂质体的表面功能化研究

项目编号： No.61305124

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 自动化技术、计算机技术

项目作者： 张立

作者单位： 香港中文大学深圳研究院

项目金额： 25万元

中文摘要： 无线操控的医疗微型机器人对于微创医疗有着革命性的应用前景，如靶向释药等。在各种驱动微型机器人的方法中,外磁场驱动方式非常有利于人体内应用，因为低强度磁场对于活体细胞和生物组织无害。人造细菌鞭毛(ABF)是一种通过磁场操控，模仿细菌运动的螺旋状微型机器人。迄今为止，虽然ABF的制备和磁控运动得到了大力发展，但对于执行体内医疗任务和细胞操作分析，其功能化问题必须得到解决。本项目旨在研究和发展ABF的表面功能化，首次提出以热敏脂质体(DPPC)来修饰ABF表面，使ABF不仅可以用于体外单细胞分析，也可进一步用于体内智能靶向释药。我们将研究ABF的表面修饰过程及机理，功能化器件的脂质体稳定性，游动特性和热敏性，以及实现单细胞荧光转染。基于脂质体可控释药的功能，本研究结果不仅将对ABF功能化提出一个新方案，而且将为发展智能微型机器人在体内生物医疗上的应用提供理论和实验基础。

中文关键词： 微型机器人；外磁场驱动；脂质体；靶向释药；单细胞微操作

英文摘要： Remotely controlled medical microrobots have the potential to revolutionize minimally invasive medical tasks, such as targeted diagnosis and therapy. Among various approaches for powering microrobots, magnetic field actuation is promising for in vivo applications, because weak-strength magnetic fields are harmless to living cells and biological tissue. Artificial bacterial flagella (ABF) are magnetic-field-driven helical microrobots, which mimic the corkscrew propulsion strategy and are of comparable size to real bacterial such as E. coli. Previous work proposed flagellar propulsion as a promising approach for nanolocomotion. ABFs are capable of precisely 3D navigation in different liquids under weak-strength magnetic fields 1000 times lower than the fields used in clinical MRI systems. While this unique technological breakthrough has many potential applications, such as targeted drug delivery, diagnosis, and implantation or removal of tissue and objects, one of the main challenges of the microrobots, i.e. the functionality, have yet to be adequately addressed. This project directly addresses this key issue that must be solved in order to realize biomedically relevant applications. Specifically, we will develop a new process for surface functionalization of ABFs using DPPC-liposome, which are capable of loading

英文关键词： microrobots；magnetic field actuation；liposome；targeted drug delivery；single-cell micromanipulation