单颗粒电致化学发光生物分析

项目名称： 单颗粒电致化学发光生物分析

项目编号： No.21475058

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 高分子科学

项目作者： 徐静娟

作者单位： 南京大学

项目金额： 90万元

中文摘要： 针对纳晶材料电致化学发光分析中存在的问题，如表面组装的不均一以及组装后纳晶粒径的不可控性带来的界面电子和能量转移效率的平均化等问题，我们提出单颗粒电致化学发光生物分析这一研究项目。拟发展能够用于单颗粒检测的纳米材料，建立形貌可控、粒径可调的纳米颗粒阵列制备技术，实现纳米材料在电极表面的精确组装和调控，揭示颗粒粒径、间隔距离、颗粒形状等对ECL的影响；深入研究基于能量转移的电致化学发光－等离子体相互作用机制，为利用纳米导线设计分子器件提供理论基础；研发基于单颗粒的生物检测技术，构建生物传感界面、设计长距离分子标尺；建立新型高灵敏的纳米传感器，探索对生物分子进行灵敏、准确的可视化检测方法，实时观察生物分子结合的过程，为生命分析科学研究提供有力的技术支撑。

中文关键词： 电化学发光；单纳米颗粒；能量传递；纳米阵列；生物传感器

英文摘要： Aiming at existing problems in electrochemiluminescence (ECL) bioanalysis, e.g., the inhomogeneity of surface assembly, the averageness of the interfacial electron and energy transfer efficiency caused by the uncontrollability of the nanocrystal's size after assembly, we propose the research project of single particle-based ECL bioanalysis. We plan to exploit nanomaterials that appropriate for single particle analysis, to develop morphology and size controllable technology for the preparation nanoparticles array, to realize the precise assembly and regulation of nanomaterials on the electrode surface, and to reveal the influences of particle size, particle shape as well as interparticle distance on ECL; In-depth study will be performed on the study of energy transfer-based ECL-plasma interaction mechanism, with the aim to provide theoretical basis for using the nanowire to design molecular devices; We will develop single particle-based bioanalysis technology, construct biological sensing interface and design of long-distance molecular ruler. We will further establish new ultrasensitive nanosensors, explore sensitive and accurate visual detection method for biomolecules and real-time observe the process of biological recognition, providing a powerful technical support for the scientific research of bioanalysis.

英文关键词： electrochemiluminescence;single nanoparticle;energy transfer;nanoparticle array;biosensor