基于光电化学的活体分析新方法研究

项目名称： 基于光电化学的活体分析新方法研究

项目编号： No.21305129

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 李改平

作者单位： 郑州大学

项目金额： 25万元

中文摘要： 生理活性分子在生命活动过程中扮演着极为重要的角色，对这些分子建立快速简便的分析方法有助于深入认识生命过程的本质，因此具有非常重要的意义。本项目拟制备无机半导体纳米管阵列，通过复合碳量子点与金纳米颗粒实现其对可见光响应并具有高的光电转换效率，利用复合纳米材料形成的三维结构提高酶的固定量并有效保持其生物活性，构建基于无机半导体纳米复合材料的光电化学生物传感器，并与活体微透析取样技术相结合，实现活体中多种生理活性物质的高灵敏高选择性检测。本项目的实施不仅开拓了多功能纳米复合材料在活体分析领域的新应用，也为活体中痕量物质的高灵敏高选择性检测提供一种新方法。

中文关键词： 传感器；光电化学；电化学；荧光；微透析

英文摘要： It is of great significance to quantitatively monitor the levels of bioactive molecules in vivo, because they play key roles in many critical biological processes. It also offers opportunities to understand the essence of biological process. In this project, we plan to develop a new visible-light-activated photoelectrochemical sensor for determination of various bioactive molecules in vivo with high sensitivity and specificity. The constructed highly ordered ZnO nanotube arrays are going to be modified with carbon quantum dots and Au nanoparticles, and it will make the whole system active under visible light illumination and improve the photon-electron conversion efficiency. Additionally, the well-connected nanostructure will offer a biocompatible microenvironment for increasing the quantity and bioactivity of immobilized enzymes, leading to high sensitivity and a fast response of the sensor. This strategy may promote the applications of new functional nanomaterials in the in vivo analysis.Moreover, the established method can provide a sensitive approach for the detection of biomolecules with very low concentration in vivo.

英文关键词： sensor；photoelectrochemistry；electrochemistry；fluorescent；microdialysis