基于FRET原理的高灵敏度DNA纳米荧光探针的合成及性能研究

项目名称： 基于FRET原理的高灵敏度DNA纳米荧光探针的合成及性能研究

项目编号： No.21201117

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 无机化学

项目作者： 刘金亮

作者单位： 上海大学

项目金额： 25万元

中文摘要： 目前，乙肝（HBV）、耐甲氧西林金黄色葡萄球菌（MRSA）已和艾滋病成为世界三大最难解决的感染性疾病，及早和准确的检测引起这些疾病的DNA序列对于乙肝及MRSA患者的诊断和治疗具有重要意义。本项目针对目前临床中乙肝及MRSA病毒DNA检测技术要求高、误差大、易出现假阳性结果等问题，设计构筑以稀土上转换发光纳米粒子作为能量给体，有机染料或金纳米粒子作为能量受体的基于荧光共振能量转移（FRET）原理的高灵敏度DNA纳米荧光探针，系统研究FRET过程相关因素的影响，建立上转换纳米粒子发光强度、粒径尺寸、能量给体与受体间距离、能量传递效率与检测灵敏度之间的相互关系，筛选出高灵敏度、高选择性识别HBV-DNA及MRSA-DNA的杂化纳米探针，实现乙肝、MRSA病毒DNA的痕量检测。本研究将对拓展上转换发光材料在生物方面的应用，扩展DNA的检测手段，及对乙肝、MRSA传染病的诊断和治疗具有重要意义。

中文关键词： 上转换发光纳米粒子；发光共振能量转移；细胞成像；DNA检测；离子检测

英文摘要： Infections caused by Hepatitis B Virus (HBV) and Methicillin-resistant Staphylococcus aureus (MRSA) have been considered the most difficult to treat, along with the AIDS disease. Early and accurate detection of the DNA sequences of the virus or bacteria is extremely important for the disease diagnosis as well as treatment. The current DNA detection methods in clinical application suffer from high cost and they are prone to false positive results. This project aims to design and construct a novel FRET-based upconversion nanoprobe for highly sensitive HBV and MRSA DNA detection. Rare-earth upconversion nanophosphors are well-known for their narrow absorption and emission bands, high chemical stability, low biological toxicity, etc. Up-conversion Nanoparticles (UCNPs) will be used as the energy donor and organic dyes or Au nanoparticle as the energy acceptor for the FRET-based nanoprobe. Systematic studies on the parameters such as the emission intensities of the UCNPs, the size of the nanoparticle, the distance between the energy donor and the acceptor will be carried out in order to achieve an efficient FRET process for DNA detection. This novel nanoprobe may lead to improved HBV and MRSA detection and extend the applications of rare-earth up-conversion nanophosphors in the biological aspects.

英文关键词： Upconversion nanoparticle；LRET；cell imaging；DNA detection；ion detection