Pax6基因和Pax6基因突变体在非洲爪蟾眼睛发育过程中的相互作用及分子机制

项目名称： Pax6基因和Pax6基因突变体在非洲爪蟾眼睛发育过程中的相互作用及分子机制

项目编号： No.31471953

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 高岚

作者单位： 兰州大学

项目金额： 87万元

中文摘要： 非洲爪蟾(Xenopus laevis)作为两栖类模式动物，在研究脊椎动物的发育以及相关基因调控、蛋白质间的相互作用等方面发挥着重要作用。本项研究以非洲爪蟾为实验材料，采用国际上先进的研究方法，通过RT-PCR、建立酵母表达系统以及免疫共沉淀实验（Co-IP），证实眼睛发育过程中Pax6 与Pax6突变基因之间的关系；并且利用TALENs技术，在无尾两栖类动物中建立Pax6基因和Pax6突变基因模型，力争揭示两者在眼睛发育过程中的相互作用，从理论上探讨相关的分子机制。 通过对眼睛发育的主导基因Pax6的深入研究，特别是对其特异性突变体基因表达的分析测定，不仅为深入研究两栖动物眼睛发育过程中分子信号通路打下坚实的基础，也有助于我们更好的了解脊椎动物发育的分子调控体系；相关研究成果也将为人类眼睛疾病及治疗的技术理论和应用提供依据。

中文关键词： 非洲爪蟾；眼睛发育；Pax6基因；Pax6突变基因；分子机制

英文摘要： Among all the amphibian model animals which are commonly introduced in developmental biology lab, Xenopus laevis is probably the most well studied species. As model species，it offers studies of the development and function of in gene level, as well as their related protein in vertebrates. In our previous studies, we studied temporal and special expression of pax6 and pax6 varient gene in the process of Xenopus laevis tadpole by gene cloning and PT-PCR for yeast 2-hybrid systenm and Co-immunoprecipitation, the aim of this study was to explore the relationship between Pax6 variant and Pax6.TALENs was also used to establish the model of the relationship between Pax6 variant and Pax6. Revealing the main role both in eye development processas well as exploring the molecular mechanism of related theory. By conducting in-depth studies of the dominant gene Pax6 , in particularly the determination and Analysis on the expression of specific variant gene in eye development, would be of great beneficial for not only the molecular process of in-depth study of amphibious animal development pathway and lay a solid foundation, but also for better understand the molecular regulation of vertebrates eye development system in the process of related; research results will provide the basis for technology theory and application of disease and treatment of human eyes.

英文关键词： Xenopus Laevis;Eye development;Paired box 6;Paired box 6 variant;Molecular mechanisms