CSP-GNPs对宫颈癌的靶向放疗增敏作用及其机制的研究

项目名称： CSP-GNPs对宫颈癌的靶向放疗增敏作用及其机制的研究

项目编号： No.81472449

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 梁晓秋

作者单位： 南华大学

项目金额： 72万元

中文摘要： 宫颈癌是女性常见恶性肿瘤，放疗是治疗宫颈癌的主要方法之一。靶向放射增敏剂将极大地提高肿瘤对放疗的敏感性，是改善放疗疗效的有效途径。但是，尚无理想的靶向放射增敏剂应用于临床。我们前期工作，首次筛选、鉴定出宫颈癌特异性结合肽(cervical cancer specific binding peptide,CSP)，并将其与纳米金（gold nanoparticles,GNPs）相连，观察到这连肽纳米金（CSP-GNPs）能明显地增强癌细胞对放射的敏感性。本项目将建立宫颈癌裸鼠移植瘤模型，验证CSP-GNPs的靶向定位效果；检测CSP-GNPs对宫颈癌细胞株的放疗增敏作用，采用移植瘤模型进行体内放疗增敏实验，并探讨其作用机制；进行CSP-GNPs在荷瘤裸鼠的药代动力学研究，对其安全性进行评估。本项目的实施有望发现新的宫颈癌靶向放射增敏剂，具有很好的应用前景，为宫颈癌治疗提供崭新思路和有益探索。

中文关键词： C22_宫颈肿瘤；宫颈癌；特异性结合肽；纳米金；靶向放疗增敏剂

英文摘要： Cervical cancer is common malignant tumors in the female. At present,Radiotherapy is a one of main method of the clinical treatment. The targeted radiosensitizer markedly enhances radiosensitization of cancer cells. Its application is an effective way for improved efficacy of Radiotherapy. But, now there were not yet suited sensitizer using to clinic. In our previous work, the specific peptide binding cervical cancer (CSP) first was selected and identified, and the peptide-gold nanoparticles compound (CSP-GNPs) were obtained. In vitro experiments, it was found that CSP-GNPs enhance radiosensitization of cancer cells. In this project, The radiosensitization of CSP-GNPs will be further detected in cervical cancer cell line and nude mice xenograft tumor model of human cervical cancer, and its mechanism will be explored. the targeting effect of CSP-GNPs will be validated by using nude mice xenograft tumor model of human cervical cancer. Pharmacokinetics of CSP-GNPs will be studied in tumor bearing nude mice. The security of CSP-GNPs will be assessed. These will offer a basis for the clinical drug research. This project is expected to find a new targeted radiosensitizer for therapy of cervical cancer, and there were good application prospects. It will provide new methods and effective exploration for treatments of cervical cancer.

英文关键词： cervical cancer;specific binding peptide;gold nanoparticles;targeted radiosensitizer