葡萄糖醛酸寡糖的酶法合成及其与朊病毒蛋白和选凝素相互作用的研究

项目名称： 葡萄糖醛酸寡糖的酶法合成及其与朊病毒蛋白和选凝素相互作用的研究

项目编号： No.30870547

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 曾晓雄

作者单位： 南京农业大学

项目金额： 30万元

中文摘要： 本项目主要研究结果如下:（1）探讨了beta-葡萄糖醛酸苷酶的底物特异性，建立了葡萄糖醛酸寡糖的酶法合成方法；（2）确立了葡萄糖醛酸寡糖酶法合成的最优条件，合成了各种葡萄糖醛酸寡糖；（3）利用糖苷酶的区域选择性，建立了结构不同的活性寡糖的酶法合成方法，并合成了各种结构不同的活性寡糖，如N-乙酰乳糖胺、N-乙酰异乳糖胺等系列寡糖；（4）确定了GOS、乳果糖、N-乙酰乳糖胺及其衍生物等的酶法合成最优条件；（5）建立了有机溶剂体系中N-乙酰己糖胺寡糖衍生物的酶法合成方法；（6）利用化学法或化学-酶法合成了各种人工合成糖高分子；（7）建立了硫辛酸衍生化糖化合物的合成方法，并利用表面等离子共振技术分析了糖与植物凝集素、朊病毒蛋白、血凝素等蛋白质的相互作用。 培养了4名博士研究生与3名硕士研究生，其中2名博士研究生的学位论文分别获得了2010年度江苏省优秀博士学位论文和2011年度江苏省优秀博士学位论文。已申请国家发明专利2项，发表SCI论文11篇。

中文关键词： 酶法合成；葡萄糖醛酸寡糖；活性寡糖；表面等离子共振；相互作用

英文摘要： The main results of the present study are as the followings: (1) Practical enzymatic synthetic procedure for oligosaccharides?containing beta-D-glucopyranuronate unit has been developed. (2) The enzymatic conditions for synthesis of oligosaccharides?containing beta-D-glucopyranuronate unit were optimized, and a series of desired oligosaccharides were synthesized. (3) Practical and convenient enzymatic synthetic procedures for functional oligosaccharides have been developed according to the regioselectivity of beta-galactosidase, and a series of functional oligosaccharides, such as N-acetyllactosamine, GOS and N-acetylisolactosamine, were synthesized. (4) The enzymatic conditions for synthesis of those oligosaccharides were obtained. (5) Enzymatic procedure for the synthesis of N-acetyllactosamine derivatives has been developed. (6) Artificial glycopolymers were synthesized by chemical or chemical-enzymatic method. (7) Interactions of oligosaccharides with lectin, prion protein and hemagglutinin of influenza virus were analyzed by using sugar-chips and surface plasmon resonance technology. Four doctoral students and three master students were graduated. Two inventions were applied Chinese patent, and 11 articles (SCI jounal) were published.

英文关键词： Enzymatic synthesis;oligosaccharides containing beta-D-glucopyranuronate;functional oligosaccharide;surface plasmon resonance;interaction