功能分析H2S调控拟南芥过氧化物酶体H2O2介导的防御响应

项目名称： 功能分析H2S调控拟南芥过氧化物酶体H2O2介导的防御响应

项目编号： No.31300225

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 韩毅

作者单位： 合肥工业大学

项目金额： 23万元

中文摘要： 植物受其移动性的限制，它通过开发自身多重应对机制来抵御环境波动。研究暗示，硫化氢（H2S）可能通过上调抗氧化系统，加速过氧化氢（H2O2）的代谢，进而调控植物对胁迫适应性与抗病性。然而H2O2、H2S与抗氧化系统之间直接的互作关系尚未明确。为解决上述问题，我们利用拟南芥过氧化氢酶缺失突变体cat2作为氧化胁迫模拟工具，研究H2S对过氧化物酶体H2O2诱导的氧化胁迫的调控作用。另一方面，尽管H2S可能被认为是一种抗氧化信号分子，但是，相关研究暗示其可能信号机制不仅于此。并且受其可能的抗氧化机制干扰，鉴定H2S作为氧化信号传递子的证据一直不是十分清晰。因此，我们将研究H2S是否能够传递H2O2信号去激活其下游防御途径。总之，本项目将利用模式研究系统去揭示植物细胞内H2O2与H2S的互作对下游防御信号途径的调控机制，为理解与优化植物响应外界胁迫干扰提供理论依据。

中文关键词： 硫化氢；过氧化氢；水杨酸；衰老；基因表达

英文摘要： Unlike animals, plants cannot move to a more comfortable location to escape stresses of external origin. Instead, plants have developed coping mechanisms that allow them to withstand environmental fluctuations. Emerging evidence implicates hydrogen sulfide (H2S) in cellular acclimation to abiotic stress as well as resistance to pathogens. It has been suggested that H2S functions in the alleviation of enviromental factors triggered oxidative damage through detoxification of hydrogen peroxide (H2O2) via the up-regulation of antioxidant systems. However, the relationship between H2O2, H2S and antioxidant systmes is not yet clearly established in plants. In order to directly investigate the possible effect of H2S on the metabolism of H2O2, a Arabidopsis mutant, cat2, which has a defect in metabolism of peroxisomal H2O2, has been used as a stress mimic tool to assess the importance of H2S that could be important in oxidative stress. On the other hand, although the 'antioxidative role' of this signaling compound has been proposed, the importance of H2S in signaling remains unclear. H2S may a candidate for transmission of signals in plant responses to oxidative stress but identification of signaling roles is complicated by its 'antioxidant function'. Thus, this study aims to establish if H2S plays an important function

英文关键词： H2S；H2O2；Salicylic acid；senscence；gene expression