苹果6-磷酸山梨醇脱氢酶基因启动子的功能分析与应用

项目名称： 苹果6-磷酸山梨醇脱氢酶基因启动子的功能分析与应用

项目编号： No.31201600

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 梁东

作者单位： 西北农林科技大学

项目金额： 23万元

中文摘要： 山梨醇是大多数蔷薇科植物主要的光合产物。山梨醇也能作为小分子渗调物质参与植物对逆境的抵御反应。6-磷酸山梨醇脱氢酶(S6PDH)是这些植物体内生成山梨醇的关键酶。前期的研究结果表明，S6PDH基因的启动子（S6PDHp）可能具有组织特异表达和胁迫诱导表达等特性。为探明S6PDHp的功能特性并加以利用，本项目拟通过将不同长度启动子缺失体转化番茄和拟南芥，通过GUS基因的表达来分析S6PDHp的组织特异表达和逆境诱导表达等特性，寻找关键的顺式作用调控区域；通过RLM-5`RACE法发现不同条件下S6PDH基因的转录起始位点；参考其他启动子的研究，通过增加一些增强子来提高S6PDHp的活性。通过该项目的实施，不仅可以探明S6PDHp的功能特性，加深对S6PDH基因表达调控的认识，还可以将S6PDHp作为一个组织特异表达和胁迫诱导表达型启动子广泛的应用于不同植物的遗传转化研究中。

中文关键词： 苹果；6-磷酸山梨醇脱氢酶；启动子；功能特性；

英文摘要： Sorbitol is the primary photosynthetic product in many species of Rosaceae. In addition, sorbitol is an efficient osmolyte. Its accumulation can improve plant tolerance against abiotic stress.NADP-dependent sorbitol-6-phosphate dehydrogenase (S6PDH)play a key role in the biosynthesis of sorbitol.In the previous study, we found that the expression of S6PDH promoter (S6PDHp)may be tissue-specific and stress-inducible. In order to find out the functional characteristics of S6PDHp and further put it into use,in this study,the 5`deletion of S6PDHp will be transformated to tomato and Arabidopsis to analysis promoter characteristics by the expression of GUS and find key regions in the promoter.The transcription initiation sites for apple S6PDH expressed in different conditions will be mapped by RLM-5`RACE.The activity of S6PDHp will be increased by adding the enhancer derived from other promoters.Through this project implementation,we can not only explore the characteristics of S6PDHp and improve our understanding on expression regulation of S6PDH,but also make use of S6PDHp as a kind of tissue-specific and stress-inducible promoter to the genetic transformation of plants.

英文关键词： Apple；Sorbitol-6-phosphate dehydrogenase；Promoter；Functional characteristics；