石墨烯在血细胞上的粘附和对细胞结构功能的影响

项目名称： 石墨烯在血细胞上的粘附和对细胞结构功能的影响

项目编号： No.21301015

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 刘佳蕙

作者单位： 北京化工大学

项目金额： 28万元

中文摘要： 作为碳纳米材料家族的新成员，石墨烯的性质及应用研究受到国内外广泛关注。尤其在生物医药领域，石墨烯及其衍生物可用于生物传感器、药物载体、显像剂、光动力治疗等，显露了极强的应用潜力。血液是石墨烯进入生物体内发挥各种生物应用潜能的必经途径之一,而石墨烯独特的单分子厚度二维微米表面，必将与同样微米尺度的血细胞相互粘附。我们推测石墨烯的粘附将通过改变血细胞表面的理化性质影响血细胞生化功能的实现。基于该假设，我们拟建立一套研究纳米材料在不同血细胞上的粘附能力及粘附对细胞结构功能影响的方法，在细胞和动物水平，研究不同尺寸、表面结构、分散体系等理化性质的石墨烯在不同类血细胞上粘附能力的差异，并进一步探讨石墨烯的粘附对不同类血细胞的完整性、变形性、运动能力以及生化功能的影响。本课题的完成将为纳米材料尤其是石墨烯的生物医学应用及安全性研究提供重要的数据支持。

中文关键词： 石墨烯；血细胞；流变学；纳米毒理学；

英文摘要： As a rising star from the carbon nanomaterial family, graphene raises great attentions in their properties and applications research.Especially in the biomedicine field, graphene and its derivates have shown huge potentials, as biosensores, nanocarriers, and imaging or photo-therapy agents. However, when graphene passes through the blood, the classical way for exnobiotics, its unique micro-size surface would attach onto the blood cells, which are at the comparible dimension. It is hypothesized that the attachment would change the complicate biological function of blood cells, by altering the physichemical characters of the surface of cells.This topic proposed to establish the methodology to detect the adhesion and the following effects between nanomaterials and blood cells. We assumpt to discover the selective and mechanism of the interaction between blood cells and graphene. How the size, surface charge and dispersing agent determine the adhesion ability of graphene on blood cells would be examined, while the integrity, deformability and biomedical functions of blood cells with graphene attachment would be tested in vitro and in vivo. This work would support the biomedical applications of graphene and researches for nanomaterials.

英文关键词： granphenen；blood cells；rheology；nanotoxicology；