聚吡咯纳米线/类骨磷灰石图案化复合材料的制备及其与成骨细胞相互作用研究

项目名称： 聚吡咯纳米线/类骨磷灰石图案化复合材料的制备及其与成骨细胞相互作用研究

项目编号： No.31271009

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 葛东涛

作者单位： 厦门大学

项目金额： 80万元

中文摘要： 材料表面的拓扑结构和外力场因素（如电刺激等）能对细胞的多种行为及生理功能产生显著影响。因此，研究经适当设计加工后的材料表面在外力场作用下对细胞的生长、发育、增殖以及功能分化等一系列事件的影响，对于制备高性能生物材料具有重要的理论和现实意义。本课题拟采用微机电加工技术构建导电高分子聚吡咯纳米线/类骨磷灰石复合图案，将材料表面的拓扑结构与电刺激相结合，系统考察图案化后的复合材料与成骨细胞之间的相互作用及作用机制，并进一步围绕其生物活性展开深入研究。预期通过本课题的实施，将获得一种具有高生物活性，并能在微纳米层次上精确有效调控成骨细胞生长的骨移植材料表面活性涂层，进而深化导电高分子纳米线这一具有优良刺激响应性能的功能材料在骨移植材料及其相关领域的应用，为开发综合性能更加优异的生物医学新产品提供重要、翔实的理论和实验依据。

中文关键词： 聚吡咯纳米线；类骨磷灰石；图案化；成骨细胞；电刺激

英文摘要： The application of electrically conductive polypyrrole (PPy) has been a focus in the field of biomedical science over the past dacades. As a bioactive coating material of bone implants, PPy could response to the electrical stimulation, which has caused great interest among researchers. It has been confirmed that PPy could regulate the cell adhesion, spreading and proliferation, and further improve the differentiation of osteoblastic cells under the external electrical stimulation. So PPy was expected to be an ideal stimuli-responsive coatings of bone implants. But unfortunately, PPy has low bioactivity.Under such backgound, the motivation of the present project is to prepare a PPy/apatite composite in order to introduce the good bioactivtiy of apatite to the final composite. It's hoped that such a composite can retain both the advantages of PPy and the high bioactivity of apatite, making it possible for the futher application in the field of bone implant materials. Furthermore, recent studies have confirmed that the topological structure of material surface has a remarkable effect on cell's behaviors and physiological processes. Thus, proper design and preparing of the material surface could control a series of events of cells, such as growth, development, proliferation and differentiation. In addition, the rapi

英文关键词： polypyrrole nanowires；apatite；micropatterning；ostroblast；electrical stimulation