拟南芥辐射旁效应信号转导的关键基因研究

项目名称： 拟南芥辐射旁效应信号转导的关键基因研究

项目编号： No.11275230

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 卞坡

作者单位： 中国科学院合肥物质科学研究院

项目金额： 80万元

中文摘要： 为解释低能离子植物诱变机制而开展的植物辐射旁效应研究，目前已经取得了一定的进展，证明辐射诱导的远程信号转导可能是低能离子诱导植物遗传变异的重要基础之一，但是目前植物辐射远程诱导的分子机制还远未清晰，参与辐射损伤信号产生、远程诱导的关键基因和信号途径还亟待阐明。本项目主要是利用拟南芥15-6#辐射旁效应实验体系简单快捷，便于批量操作的特点，和拟南芥成熟的T-DNA插入突变技术有机结合，构建辐射旁效应相关基因的快速筛选体系。拟通过大量的筛选和后续的遗传分析，定位、克隆部分参与植物辐射旁效应的相关基因。在此基础上使用拟南芥根局部辐射微嫁接技术确定目的基因参与植物辐射旁效应的过程。最后借助拟南芥遗传信息资源库和生物途径数据库，关联目的基因的生物学功能及其所属的信号通路，构建一个植物辐射旁效应信号转导通路的初步框架，为更深入的研究低能离子诱变的内在分子机理奠定基础。

中文关键词： 辐射旁效应；茉莉酸；水杨酸；微重力；拟南芥

英文摘要： The radiation-induced bystander effect in vivo in plants was carried out for the understanding of the low-energy-ion mutagenesis. The distant signal transduction was reported to be implicated in the low-energy-ion mutagenesis in plants, whereas the underlying molecular mechanism is so far clear. At present, it is necessary to isolate and characterize some genes involved in the generation of bystander signal(s) and the bystander responses. In this proposal, a T-DNA insert mutant library is first established, with the Arabidopsis 15-6# line transgenic for AtRAD54 promoter::GUS as the genetic background, which was widely used in the analysis of bystander effects in vivo in plants. Due to the brief experimental process of the 15-6# line, the function-loss mutants in bystander effect can be screened rapidly from the T-DNA insertion library. After isolating some of the related mutants, their wildtype genes will be then determined to take part in the gneration of signal(s) in irradiated tissues or distant induction of effect in bystander tissues, or both, using the Arabidopsis root micro-grafting. Finally, a primary outline regarding the signaling pathways of bystander effect in plants will be drawn based on the biological function of the isolated genes and their roles in signal transduction. The work will be signific

英文关键词： radiation induced bystander effects；jasmonic acid；salicylic acid；microgravity；Arabidopsis thaliana