苹果ACC合成酶基因MdACS6在果实乙烯合成中的调控机制研究

项目名称： 苹果ACC合成酶基因MdACS6在果实乙烯合成中的调控机制研究

项目编号： No.31201601

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 王爱德

作者单位： 沈阳农业大学

项目金额： 25万元

中文摘要： 植物体中乙烯合成分为系统1和系统2。ACC合成酶（ACS）是乙烯信号途径中调控呼吸跃变型果实成熟的关键酶，从苹果全基因组数据库中获得果实成熟相关的新ACS基因，命名为MdACS6。MdACS6从果实采收前60天开始表达，并在室温贮藏过程中逐渐减弱，初步推断MdACS6可能作用于系统1乙烯合成。本项目在此基础上，首先通过RACE法克隆MdACS6基因的全长序列，明确其组织特异性和时空表达情况，结合MdACS6在室温贮藏和1-MCP（乙烯抑制剂）及乙烯处理的苹果果实中的表达情况，确定其是否为乙烯合成系统。通过Western blot 检测其在果实不同发育阶段的蛋白水平，通过农杆菌注射实验在未成熟苹果果实中过表达MdACS6以及番茄转基因实验，研究MdACS6在苹果果实乙烯合成系统1和系统2中的功能；阐明MdACS6在调控苹果果实乙烯合成和成熟中的作用，完善果实成熟理论，为果实贮藏提供理论依据。

中文关键词： 苹果；果实成熟；乙烯；ACS；调控

英文摘要： Ethylene biosynthesis in plant is divided into two systems, system 1 and system 2. ACC (1-Aminocyclopropane-1-carboxylic acid) synthase (ACS) is the most important enzyme in ethylene signaling pathway regulating the ripening of climacteric fruit. A new member of apple ACS family, named MdACS6, was found by searching the apple genome database. MdACS6 is expressed differentially in apple fruit development and ripening, which started from 60 d before commercial harvest and decreased during the storage at room temperature. We propose that MdACS6 might work in system 1 ethylene synthesis. In this proposal, the following experiments are designed to study the role of MdACS6 in regulating system 1 and system 2 ethylene synthesis in apple fruit based upon these findings. Firstly, the full length of MdACS6 will be obtained by RACE. Its expression during apple fruit development and in different organisms will be detected by real time PCR. Fruits will be treated with 1-MCP (1-Methylcyclopropene, an ethylene inhibitor) and ethylene, which will be used to check the expression of MdACS6 to determine whether it is affected by ethylene. The protein of MdACS6 will also be detected by Western blot assay in different developmental stage and during storage in air. An assay of Agrobacterium infiltration will be carried out on unripe

英文关键词： apple；fruit ripening；ethylene；ACS；regulation